ΔFosB induction in striatal medium spiny neuron subtypes in response to chronic pharmacological, emotional, and optogenetic stimuli

Mary Kay Lobo, Samir Zaman, Diane M. Damez-Werno, Ja Wook Koo, Rosemary C. Bagot, Jennifer A. DiNieri, Alexandria Nugent, Eric Finkel, Dipesh Chaudhury, Ramesh Chandra, Efrain Riberio, Jacqui Rabkin, Ezekiell Mouzon, Roger Cachope, Joseph F. Cheer, Ming Hu Han, David M. Dietz, David W. Self, Yasmin L. Hurd, Vincent VialouEric J. Nestler

Research output: Contribution to journalArticle

Abstract

The transcription factor, ΔFosB, is robustly and persistently induced in striatum by several chronic stimuli, such as drugs of abuse, antipsychotic drugs, natural rewards, and stress. However, very few studies have examined the degree ofΔFosB induction in the two striatal medium spiny neuron (MSN) subtypes.Wemake use of fluorescent reporter BAC transgenic mice to evaluate induction ofΔFosB in dopamine receptor 1 (D1) enriched and dopamine receptor 2 (D2) enriched MSNs in ventral striatum, nucleus accumbens (NAc) shell and core, and in dorsal striatum (dStr) after chronic exposure to several drugs of abuse including cocaine, ethanol, Δ(9)- tetrahydrocannabinol, and opiates; the antipsychotic drug, haloperidol; juvenile enrichment; sucrose drinking; calorie restriction; the serotonin selective reuptake inhibitor antidepressant, fluoxetine; and social defeat stress. Our findings demonstrate that chronic exposure to many stimuli inducesΔFosB in an MSN-subtype selective pattern across all three striatal regions. To explore the circuit-mediated induction of ΔFosB in striatum, we use optogenetics to enhance activity in limbic brain regions that send synaptic inputs to NAc; these regions include the ventral tegmental area and several glutamatergic afferent regions: medial prefrontal cortex, amygdala, and ventral hippocampus. These optogenetic conditions lead to highly distinct patterns of ΔFosB induction in MSN subtypes in NAc core and shell. Together, these findings establish selective patterns of ΔFosB induction in striatal MSN subtypes in response to chronic stimuli and provide novel insight into the circuit-level mechanisms of ΔFosB induction in striatum.

Original languageEnglish (US)
Pages (from-to)18381-18395
Number of pages15
JournalJournal of Neuroscience
Volume33
Issue number47
DOIs
StatePublished - 2013

ASJC Scopus subject areas

  • Neuroscience(all)

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    Lobo, M. K., Zaman, S., Damez-Werno, D. M., Koo, J. W., Bagot, R. C., DiNieri, J. A., Nugent, A., Finkel, E., Chaudhury, D., Chandra, R., Riberio, E., Rabkin, J., Mouzon, E., Cachope, R., Cheer, J. F., Han, M. H., Dietz, D. M., Self, D. W., Hurd, Y. L., ... Nestler, E. J. (2013). ΔFosB induction in striatal medium spiny neuron subtypes in response to chronic pharmacological, emotional, and optogenetic stimuli. Journal of Neuroscience, 33(47), 18381-18395. https://doi.org/10.1523/JNEUROSCI.1875-13.2013