[10] 5-Fluoroorotic acid as a selective agent in yeast molecular genetics

Jef D. Boeke, Joshua Trueheart, Georges Natsoulis, Gerald R. Fink

    Research output: Contribution to journalArticle

    Abstract

    5-FOA is an extremely useful reagent for the selection of Ura- cells amid a population of Ura+ cells. The selection is effective in transformation and recombination studies where loss of URA3+ is desired. A new plasmid shuffling procedure based on the 5-FOAR selection permits the recovery of conditional lethal mutations in cloned genes that encode vital functions.

    Original languageEnglish (US)
    Pages (from-to)164-175
    Number of pages12
    JournalMethods in enzymology
    Volume154
    Issue numberC
    DOIs
    StatePublished - Dec 1 1987

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology

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