16S rRNA amplicon sequencing of urban prokaryotic communities in the South Bronx River estuary

Eugenia Naro-Maciel; Melissa R. Ingala; Irena E. Werner; Allison M. Fitzgerald

doi:10.1128/MRA.00182-20

16S rRNA amplicon sequencing of urban prokaryotic communities in the South Bronx River estuary

Eugenia Naro-Maciel, Melissa R. Ingala, Irena E. Werner, Allison M. Fitzgerald

Urban Initiative

Research output: Contribution to journal › Article › peer-review

Abstract

Biodiversity monitoring is an essential component of restoration efforts. We sequenced 16S rRNA gene amplicons from sediments and waters of Hunts Point Riverside Park and Soundview Park, located in a historically degraded but recovering urban estuary in New York. In total, 16,165 unique amplicon sequence variants were recovered, and Proteobacteria was the dominant phylum.

Original language	English (US)
Article number	e00182-20
Journal	Microbiology Resource Announcements
Volume	9
Issue number	22
DOIs	https://doi.org/10.1128/MRA.00182-20
State	Published - May 28 2020

ASJC Scopus subject areas

Molecular Biology
Immunology and Microbiology (miscellaneous)
Genetics

Access to Document

10.1128/MRA.00182-20

Cite this

@article{50167fdcd0a149ff86bef63a41202520,

title = "16S rRNA amplicon sequencing of urban prokaryotic communities in the South Bronx River estuary",

abstract = "Biodiversity monitoring is an essential component of restoration efforts. We sequenced 16S rRNA gene amplicons from sediments and waters of Hunts Point Riverside Park and Soundview Park, located in a historically degraded but recovering urban estuary in New York. In total, 16,165 unique amplicon sequence variants were recovered, and Proteobacteria was the dominant phylum.",

author = "Eugenia Naro-Maciel and Ingala, {Melissa R.} and Werner, {Irena E.} and Fitzgerald, {Allison M.}",

note = "Funding Information: We thank the New York University Research Challenge Fund (to E.N.-M.) and Experiment.com private donors (to I.E.W.) for funding the research. Special thanks are extended to Brendan Reid, as well as to student assistants Christian Bojorquez, NaVonna Turner, Sean Thomas, Jennifer Servis, and Patrick Shea. We thank the NY/NJ Baykeeper and NYC Parks (Natural Resources Group) for access to Soundview Park and Hunts Point Riverside Park, as well as for field assistance. E.N.-M. and I.E.W. developed the research question. I.E.W. and A.M.F. collected the samples and prepared them for sequencing. E.N.-M. and M.R.I. analyzed the data and wrote the paper. All the authors edited and revised the paper. Publisher Copyright: Copyright {\textcopyright} 2020 Naro-Maciel et al.",

year = "2020",

month = may,

day = "28",

doi = "10.1128/MRA.00182-20",

language = "English (US)",

volume = "9",

journal = "Microbiology Resource Announcements",

issn = "2169-8287",

publisher = "American Society for Microbiology",

number = "22",

}

TY - JOUR

T1 - 16S rRNA amplicon sequencing of urban prokaryotic communities in the South Bronx River estuary

AU - Naro-Maciel, Eugenia

AU - Ingala, Melissa R.

AU - Werner, Irena E.

AU - Fitzgerald, Allison M.

N1 - Funding Information: We thank the New York University Research Challenge Fund (to E.N.-M.) and Experiment.com private donors (to I.E.W.) for funding the research. Special thanks are extended to Brendan Reid, as well as to student assistants Christian Bojorquez, NaVonna Turner, Sean Thomas, Jennifer Servis, and Patrick Shea. We thank the NY/NJ Baykeeper and NYC Parks (Natural Resources Group) for access to Soundview Park and Hunts Point Riverside Park, as well as for field assistance. E.N.-M. and I.E.W. developed the research question. I.E.W. and A.M.F. collected the samples and prepared them for sequencing. E.N.-M. and M.R.I. analyzed the data and wrote the paper. All the authors edited and revised the paper. Publisher Copyright: Copyright © 2020 Naro-Maciel et al.

PY - 2020/5/28

Y1 - 2020/5/28

N2 - Biodiversity monitoring is an essential component of restoration efforts. We sequenced 16S rRNA gene amplicons from sediments and waters of Hunts Point Riverside Park and Soundview Park, located in a historically degraded but recovering urban estuary in New York. In total, 16,165 unique amplicon sequence variants were recovered, and Proteobacteria was the dominant phylum.

AB - Biodiversity monitoring is an essential component of restoration efforts. We sequenced 16S rRNA gene amplicons from sediments and waters of Hunts Point Riverside Park and Soundview Park, located in a historically degraded but recovering urban estuary in New York. In total, 16,165 unique amplicon sequence variants were recovered, and Proteobacteria was the dominant phylum.

UR - http://www.scopus.com/inward/record.url?scp=85086732604&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85086732604&partnerID=8YFLogxK

U2 - 10.1128/MRA.00182-20

DO - 10.1128/MRA.00182-20

M3 - Article

AN - SCOPUS:85086732604

SN - 2169-8287

VL - 9

JO - Microbiology Resource Announcements

JF - Microbiology Resource Announcements

IS - 22

M1 - e00182-20

ER -

16S rRNA amplicon sequencing of urban prokaryotic communities in the South Bronx River estuary

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this