3D arrays for high throughput assay of cell migration and electrotaxis

Sanjun Zhao; Runchi Gao; Peter N. Devreotes; Alex Mogilner; Min Zhao

doi:10.1002/cbin.10116

3D arrays for high throughput assay of cell migration and electrotaxis

Sanjun Zhao, Runchi Gao, Peter N. Devreotes, Alex Mogilner, Min Zhao

Research output: Contribution to journal › Article › peer-review

Abstract

Cell behaviour in 3D environments can be significantly different from those in 2D cultures. With many different 3D matrices being developed and many experimental modalities used to modulate cell behaviour in 3D, it is necessary to develop high throughput techniques to study behaviour in 3D. We report on a 3D array on slide and have adapted this to our electrotaxis chamber, thereby offering a novel approach to quantify cellular responses to electric fields (EFs) in 3D conditions, in different matrices, with different strains of cells, under various field strengths. These developments used Dictyostelium cells to illustrate possible applications and limitations.

Original language	English (US)
Pages (from-to)	995-1002
Number of pages	8
Journal	Cell Biology International
Volume	37
Issue number	9
DOIs	https://doi.org/10.1002/cbin.10116
State	Published - Sep 2013

Keywords

3D matrices
Array
Assay
Cell locomotion
Electrotaxis
Morphology

ASJC Scopus subject areas

Cell Biology

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10.1002/cbin.10116

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KW - Assay

KW - Cell locomotion

KW - Electrotaxis

KW - Morphology

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3D arrays for high throughput assay of cell migration and electrotaxis

Abstract

Keywords

ASJC Scopus subject areas

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