@article{4292bd9389e94f67aea5423e31219aad,
title = "3D bioprinting and microscale organization of vascularized tissue constructs using collagen-based bioink",
abstract = "Bioprinting three-dimensional (3D) tissue equivalents have progressed tremendously over the last decade. 3D bioprinting is currently being employed to develop larger and more physiologic tissues, and it is of particular interest to generate vasculature in biofabricated tissues to aid better perfusion and transport of nutrition. Having an advantage over manual culture systems by bringing together biological scaffold materials and cells in precise 3D spatial orientation, bioprinting could assist in placing endothelial cells in specific spatial locations within a 3D matrix to promote vessel formation at these predefined areas. Hence, in the present study, we investigated the use of bioprinting to generate tissue-level capillary-like networks in biofabricated tissue constructs. First, we developed a bioink using collagen type-1 supplemented with xanthan gum (XG) as a thickening agent. Using a commercial extrusion-based multi-head bioprinter and collagen-XG bioink, the component cells were spatially assembled, wherein the endothelial cells were bioprinted in a lattice pattern and sandwiched between bioprinted fibroblasts layers. 3D bioprinted constructs thus generated were stable, and maintained structural shape and form. Post-print culture of the bioprinted tissues resulted in endothelial sprouting and formation of interconnected capillary-like networks within the lattice pattern and between the fibroblast layers. Bioprinter-assisted spatial placement of endothelial cells resulted in fabrication of patterned prevascularized constructs that enable potential regenerative applications in the future.",
keywords = "additive manufacturing, bioink, bioprinting, stem cells, tissue engineering, vascularized tissue",
author = "Senthilkumar Muthusamy and Sathya Kannan and Marcus Lee and Vijayavenkataraman Sanjairaj and Lu, {Wen Feng} and Fuh, {Jerry Y.H.} and Gopu Sriram and Tong Cao",
note = "Funding Information: The authors would like to acknowledge Lee Shu Ying from the Confocal Microscopy Unit and Yong Loo Lin School of Medicine, the National University of Singapore for her help in confocal microscopy. We also thank Giridharan Muniraj and Natu Vaishali Prakash from the Faculty of Dentistry, Wu Bin, and Harish K. Handral from the Faculty of Engineering, the National University of Singapore for their support in experimental procedures. The authors also thank the NUS Centre for Additive Manufacturing (AM.NUS), Singapore for providing access and expertise related to 3D printing. This study was supported by funds from the National Additive Manufacturing Innovation Cluster (NAMIC) (Project ID: 2017068) and the Agency for Science, Technology, and Research (A∗STAR, Singapore) Advanced Manufacturing and Engineering Individual Research Grant (AME IRG) (Project ID: A1883c0013). Funding Information: The authors would like to acknowledge Lee Shu Ying from the Confocal Microscopy Unit and Yong Loo Lin School of Medicine, the National University of Singapore for her help in confocal microscopy. We also thank Giridharan Muniraj and Natu Vaishali Prakash from the Faculty of Dentistry, Wu Bin, and Harish K. Handral from the Faculty of Engineering, the National University of Singapore for their support in experimental procedures. The authors also thank the NUS Centre for Additive Manufacturing (AM.NUS), Singapore for providing access and expertise related to 3D printing. This study was supported by funds from the National Additive Manufacturing Innovation Cluster (NAMIC) (Project ID: 2017068) and the Agency for Science, Technology, and Research (A?STAR, Singapore) Advanced Manufacturing and Engineering Individual Research Grant (AME IRG) (Project ID: A1883c0013). Publisher Copyright: {\textcopyright} 2021 Wiley Periodicals LLC.",
year = "2021",
month = aug,
doi = "10.1002/bit.27838",
language = "English (US)",
volume = "118",
pages = "3150--3163",
journal = "Biotechnology and Bioengineering",
issn = "0006-3592",
publisher = "Wiley-VCH Verlag",
number = "8",
}