TY - GEN
T1 - 3D-printed herringbone micro-mixers for immuno-capture of cancer cells
AU - Sukumar, Pavithra
AU - Deliorman, Muhammedin
AU - Brimmo, Ayoola
AU - Alnemari, Roaa
AU - Qasaimeh, Mohammad A.
N1 - Publisher Copyright:
Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - This work presents a method for fabricating herringbone micro-mixers using stereo-lithographic 3D printing for scalable, versatile, and efficient capture of cancer cells from peripheral blood samples. The 3D-printed herringbones were confirmed to create chaotic mixing patterns within the channel, both by 3D finite element analysis and experiments. Experiments showed ∼61% capture efficiency of MCF-7 breast cancer cells using immobilized anti-EpCAM antibodies. Simulations also suggested that 2- and 5× scaled herringbone micro-mixers can be used for high throughput applications. Fabricating scaled-up versions of herringbone micro-mixers can be achieved easily by modifying, on demand, CAD design before 3D printing and PDMS molding.
AB - This work presents a method for fabricating herringbone micro-mixers using stereo-lithographic 3D printing for scalable, versatile, and efficient capture of cancer cells from peripheral blood samples. The 3D-printed herringbones were confirmed to create chaotic mixing patterns within the channel, both by 3D finite element analysis and experiments. Experiments showed ∼61% capture efficiency of MCF-7 breast cancer cells using immobilized anti-EpCAM antibodies. Simulations also suggested that 2- and 5× scaled herringbone micro-mixers can be used for high throughput applications. Fabricating scaled-up versions of herringbone micro-mixers can be achieved easily by modifying, on demand, CAD design before 3D printing and PDMS molding.
KW - 3D printing
KW - Circulating tumor cells
KW - Herringbone micro-mixers
KW - Liquid biopsy
KW - Microfluidics
UR - http://www.scopus.com/inward/record.url?scp=85079751385&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85079751385&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85079751385
T3 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
SP - 2441
EP - 2442
BT - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PB - Chemical and Biological Microsystems Society
T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Y2 - 11 November 2018 through 15 November 2018
ER -