3D Printed Microfluidic Probes

Ayoola Brimmo, Pierre Alexandre Goyette, Roaa Alnemari, Thomas Gervais, Mohammad Qasaimeh

Research output: Contribution to journalArticle

Abstract

In this work, we fabricate microfluidic probes (MFPs) in a single step by stereolithographic 3D printing and benchmark their performance with standard MFPs fabricated via glass or silicon micromachining. Two research teams join forces to introduce two independent designs and fabrication protocols, using different equipment. Both strategies adopted are inexpensive and simple (they only require a stereolithography printer) and are highly customizable. Flow characterization is performed by reproducing previously published microfluidic dipolar and microfluidic quadrupolar reagent delivery profiles which are compared to the expected results from numerical simulations and scaling laws. Results show that, for most MFP applications, printer resolution artifacts have negligible impact on probe operation, reagent pattern formation, and cell staining results. Thus, any research group with a moderate resolution (≤100 µm) stereolithography printer will be able to fabricate the MFPs and use them for processing cells, or generating microfluidic concentration gradients. MFP fabrication involved glass and/or silicon micromachining, or polymer micromolding, in every previously published article on the topic. We therefore believe that 3D printed MFPs is poised to democratize this technology. We contribute to initiate this trend by making our CAD files available for the readers to test our “print & probe” approach using their own stereolithographic 3D printers.

Original languageEnglish (US)
Article number10995
JournalScientific Reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018

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Microfluidics
Printers (computer)
Stereolithography
Micromachining
3D printers
Fabrication
Glass
Silicon
Scaling laws
Printing
Computer aided design
Computer simulation
Polymers

ASJC Scopus subject areas

  • General

Cite this

Brimmo, A., Goyette, P. A., Alnemari, R., Gervais, T., & Qasaimeh, M. (2018). 3D Printed Microfluidic Probes. Scientific Reports, 8(1), [10995]. https://doi.org/10.1038/s41598-018-29304-x

3D Printed Microfluidic Probes. / Brimmo, Ayoola; Goyette, Pierre Alexandre; Alnemari, Roaa; Gervais, Thomas; Qasaimeh, Mohammad.

In: Scientific Reports, Vol. 8, No. 1, 10995, 01.12.2018.

Research output: Contribution to journalArticle

Brimmo, A, Goyette, PA, Alnemari, R, Gervais, T & Qasaimeh, M 2018, '3D Printed Microfluidic Probes' Scientific Reports, vol. 8, no. 1, 10995. https://doi.org/10.1038/s41598-018-29304-x
Brimmo A, Goyette PA, Alnemari R, Gervais T, Qasaimeh M. 3D Printed Microfluidic Probes. Scientific Reports. 2018 Dec 1;8(1). 10995. https://doi.org/10.1038/s41598-018-29304-x
Brimmo, Ayoola ; Goyette, Pierre Alexandre ; Alnemari, Roaa ; Gervais, Thomas ; Qasaimeh, Mohammad. / 3D Printed Microfluidic Probes. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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