3D Printed Microfluidic Probes

Ayoola Brimmo, Pierre Alexandre Goyette, Roaa Alnemari, Thomas Gervais, Mohammad Qasaimeh

    Research output: Contribution to journalArticle

    Abstract

    In this work, we fabricate microfluidic probes (MFPs) in a single step by stereolithographic 3D printing and benchmark their performance with standard MFPs fabricated via glass or silicon micromachining. Two research teams join forces to introduce two independent designs and fabrication protocols, using different equipment. Both strategies adopted are inexpensive and simple (they only require a stereolithography printer) and are highly customizable. Flow characterization is performed by reproducing previously published microfluidic dipolar and microfluidic quadrupolar reagent delivery profiles which are compared to the expected results from numerical simulations and scaling laws. Results show that, for most MFP applications, printer resolution artifacts have negligible impact on probe operation, reagent pattern formation, and cell staining results. Thus, any research group with a moderate resolution (≤100 µm) stereolithography printer will be able to fabricate the MFPs and use them for processing cells, or generating microfluidic concentration gradients. MFP fabrication involved glass and/or silicon micromachining, or polymer micromolding, in every previously published article on the topic. We therefore believe that 3D printed MFPs is poised to democratize this technology. We contribute to initiate this trend by making our CAD files available for the readers to test our “print & probe” approach using their own stereolithographic 3D printers.

    LanguageEnglish (US)
    Article number10995
    JournalScientific Reports
    Volume8
    Issue number1
    DOIs
    StatePublished - Dec 1 2018

    Fingerprint

    Microfluidics
    Printers (computer)
    Stereolithography
    Micromachining
    3D printers
    Fabrication
    Glass
    Silicon
    Scaling laws
    Printing
    Computer aided design
    Computer simulation
    Polymers
    Processing

    ASJC Scopus subject areas

    • General

    Cite this

    Brimmo, A., Goyette, P. A., Alnemari, R., Gervais, T., & Qasaimeh, M. (2018). 3D Printed Microfluidic Probes. Scientific Reports, 8(1), [10995]. https://doi.org/10.1038/s41598-018-29304-x

    3D Printed Microfluidic Probes. / Brimmo, Ayoola; Goyette, Pierre Alexandre; Alnemari, Roaa; Gervais, Thomas; Qasaimeh, Mohammad.

    In: Scientific Reports, Vol. 8, No. 1, 10995, 01.12.2018.

    Research output: Contribution to journalArticle

    Brimmo, A, Goyette, PA, Alnemari, R, Gervais, T & Qasaimeh, M 2018, '3D Printed Microfluidic Probes' Scientific Reports, vol. 8, no. 1, 10995. https://doi.org/10.1038/s41598-018-29304-x
    Brimmo A, Goyette PA, Alnemari R, Gervais T, Qasaimeh M. 3D Printed Microfluidic Probes. Scientific Reports. 2018 Dec 1;8(1). 10995. https://doi.org/10.1038/s41598-018-29304-x
    Brimmo, Ayoola ; Goyette, Pierre Alexandre ; Alnemari, Roaa ; Gervais, Thomas ; Qasaimeh, Mohammad. / 3D Printed Microfluidic Probes. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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