5-HT1A receptor expression is modulated by corticosteroid receptor agonists in primary rat hippocampal culture

Mayumi Nishi, Efrain C. Azmitia

Research output: Contribution to journalArticlepeer-review

Abstract

The effects of corticosteroid receptor agonists on the expression of 5-HT1A receptor mRNA were measured in rat hippocampal cultures using in situ hybridization histochemistry. In our normal culture system, grown in serum and steroid-free media, moderate to heavy signal for 5-HT1A mRNA transcripts were detected in hippocampal neurons and glial cells. Aldosterone, a type I corticosteroid receptor agonist (10-9 M), significantly reduced the expression of 5-HT1A mRNA both in neurons and glial fibrillary acidic protein (GFAP)-immuno-reactive (IR) cells. The type II corticosteroid receptor agonist, Ru28362 (10-8 M), also significantly decreased neuronal 5-HT1A mRNA expression. However, it was not as effective as aldosterone in reducing the label over GFAP-IR cells. These data indicate that corticosteroids may directly regulate the expression of hippocampal 5-HT1A receptors at the mRNA level in cultured hippocampal cells.

Original languageEnglish (US)
Pages (from-to)190-194
Number of pages5
JournalBrain Research
Volume722
Issue number1-2
DOIs
StatePublished - May 25 1996

Keywords

  • Aldosterone
  • Differentiation
  • Hybridization, in situ: mRNA
  • Ru28362
  • Tissue culture

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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