A bulky DNA lesion derived from a highly potent polycyclic aromatic tumorigen stabilizes nucleosome core particle structure

Yuqin Cai, Lihua Wang, Shuang Ding, Adam Schwaid, Nicholas E. Geacintov, Suse Broyde

Research output: Contribution to journalArticlepeer-review

Abstract

The impact of a bulky DNA lesion on the structure and dynamics of a nucleosome core particle (NCP) containing a lesion derived from the unusually potent tumorigen dibenzo[a,l]pyrene that resists nucleotide excision repair (NER) in free DNA was investigated using 65 ns molecular dynamics simulations. Our results reveal that, relative to unmodified NCP, the lesion stabilizes the nucleosome via stacking interactions, improved Watson-Crick base pairing, hydrogen bonding between DNA and histones, and damped dynamics. These findings suggest that such lesions should be as resistant to NER in the nucleosome environment as they are in free DNA.

Original languageEnglish (US)
Pages (from-to)9943-9945
Number of pages3
JournalBiochemistry
Volume49
Issue number46
DOIs
StatePublished - Nov 23 2010

ASJC Scopus subject areas

  • Biochemistry

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