A cellular FRET-based sensor for β-O-GIcNAc, a dynamic carbohydrate modification involved in signaling

Luz D. Carrillo, Lakshmipriya Krishnamoorthy, Lara K. Mahal

Research output: Contribution to journalArticlepeer-review

Abstract

β-O-N-Acetyl-d-glucosamine (O-GlcNAc) is a dynamic carbohydrate modification that is involved in cell signaling and has been implicated in a variety of disease states, including Alzheimer's and type-II diabetes. Despite the importance of this modification, little is known about the spatial and temporal localization of O-GlcNAc during signaling. This is due to the lack of methods for the study of O-GlcNAc in living cell systems. Herein we report the first genetically encoded FRET-based sensor for the detection of O-GlcNAc dynamics in live mammalian cells.

Original languageEnglish (US)
Pages (from-to)14768-14769
Number of pages2
JournalJournal of the American Chemical Society
Volume128
Issue number46
DOIs
StatePublished - Nov 22 2006

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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