A cellular FRET-based sensor for β-O-GIcNAc, a dynamic carbohydrate modification involved in signaling

Luz D. Carrillo; Lakshmipriya Krishnamoorthy; Lara K. Mahal

doi:10.1021/ja065835+

A cellular FRET-based sensor for β-O-GIcNAc, a dynamic carbohydrate modification involved in signaling

Luz D. Carrillo, Lakshmipriya Krishnamoorthy, Lara K. Mahal

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

β-O-N-Acetyl-d-glucosamine (O-GlcNAc) is a dynamic carbohydrate modification that is involved in cell signaling and has been implicated in a variety of disease states, including Alzheimer's and type-II diabetes. Despite the importance of this modification, little is known about the spatial and temporal localization of O-GlcNAc during signaling. This is due to the lack of methods for the study of O-GlcNAc in living cell systems. Herein we report the first genetically encoded FRET-based sensor for the detection of O-GlcNAc dynamics in live mammalian cells.

Original language	English (US)
Pages (from-to)	14768-14769
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	128
Issue number	46
DOIs	https://doi.org/10.1021/ja065835+
State	Published - Nov 22 2006

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

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10.1021/ja065835+

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abstract = "β-O-N-Acetyl-d-glucosamine (O-GlcNAc) is a dynamic carbohydrate modification that is involved in cell signaling and has been implicated in a variety of disease states, including Alzheimer's and type-II diabetes. Despite the importance of this modification, little is known about the spatial and temporal localization of O-GlcNAc during signaling. This is due to the lack of methods for the study of O-GlcNAc in living cell systems. Herein we report the first genetically encoded FRET-based sensor for the detection of O-GlcNAc dynamics in live mammalian cells.",

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AU - Mahal, Lara K.

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AB - β-O-N-Acetyl-d-glucosamine (O-GlcNAc) is a dynamic carbohydrate modification that is involved in cell signaling and has been implicated in a variety of disease states, including Alzheimer's and type-II diabetes. Despite the importance of this modification, little is known about the spatial and temporal localization of O-GlcNAc during signaling. This is due to the lack of methods for the study of O-GlcNAc in living cell systems. Herein we report the first genetically encoded FRET-based sensor for the detection of O-GlcNAc dynamics in live mammalian cells.

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A cellular FRET-based sensor for β-O-GIcNAc, a dynamic carbohydrate modification involved in signaling

Abstract

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