A chloroquine resistance locus in the rodent malaria parasite Plasmodium chabaudi

Jane Carlton, Margaret Mackinnon, David Walliker

Research output: Contribution to journalArticle

Abstract

We have located a possible chloroquine resistance locus in the genome of the rodent malaria parasite Plasmodium chabaudi. Two genetically distinct clones of the parasite were grown in vivo and allowed to undergo genetic crossing. The clones differed from each other in their susceptibility to chloroquine; AS(3CQ) had been selected for a low level of resistance to the drug whereas AJ is chloroquine-sensitive. Independent recombinant progeny (20) were cloned from the products of two crosses, phenotyped for their susceptibility to chloroquine, and genotyped for their inheritance of 46 chromosome-specific markers. No association was found between chloroquine susceptibility and the inheritance of pcmdr1, the P. chabaudi homologue of the pfmdr1 multi-drug resistance gene of P. falciparum. Also, there was no association between chloroquine susceptibility and the inheritance of a marker linked to a putative chloroquine resistance locus in a P. falciparum cross. However, 16 of the progeny clones showed co-segregation of four linked markers on chromosome 11 with their resistance phenotype. This result suggests that a locus for chloroquine resistance exists on this chromosome in P. chabaudi. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)57-72
Number of pages16
JournalMolecular and Biochemical Parasitology
Volume93
Issue number1
DOIs
StatePublished - May 15 1998

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Keywords

  • Chloroquine
  • Plasmodium chabaudi
  • Resistance

ASJC Scopus subject areas

  • Parasitology
  • Molecular Biology

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