TY - JOUR
T1 - A collagen membrane containing osteogenic protein-1 facilitates bone regeneration in a rat mandibular bone defect
AU - Ozaki, Manami
AU - Takayama, Tadahiro
AU - Yamamoto, Takanobu
AU - Ozawa, Yasumasa
AU - Nagao, Mayu
AU - Tanabe, Natsuko
AU - Nakajima, Akira
AU - Suzuki, Naoto
AU - Maeno, Masao
AU - Yamano, Seiichi
AU - Sato, Shuichi
PY - 2017/12
Y1 - 2017/12
N2 - Objectives Osteogenic protein-1 (OP-1) has shown osteoinductive activities and is useful for clinical treatments, including bone regeneration. Regenerative procedures using a bioabsorbable collagen membrane (BCM) are well established in periodontal and implant dentistry. We evaluated the subsequent effects of the BCM in combination with OP-1 on bone regeneration in a rat mandibular circular critical-sized bone defect in vivo. Design We used 8 rats that received surgery in both sides of the mandible, and created the total 16 defects which were divided into 4 groups: Group 1; no treatment, as a control, Group 2; BCM alone, Group 3; BCM containing low dose 0.5 μg of OP-1 (L-OP-1), and Group 4; BCM containing high dose 2.0 μg of OP-1 (H-OP-1). Newly formed bone was evaluated by micro computed tomography (micro-CT) and histological analyses at 8 weeks postoperatively. In quantitative and qualitative micro-CT analyses of the volume of new bone formation, bone density, and percentage of new bone area was evaluated. Results BCM with rhOP-1 significantly increased and accelerated bone volume, bone mineral density, and percentage of new bone area compared to control and BCM alone at 8 weeks after surgery; these enhancements in bone regeneration in the OP-1-treated groups were dose-dependent. Conclusions OP-1 delivered with a BCM may have effective osteoinductive potency and be a good combination for bone regeneration. The use of such a combination device for osteogenesis may result in safer and more predictable bone regenerative outcomes in the future.
AB - Objectives Osteogenic protein-1 (OP-1) has shown osteoinductive activities and is useful for clinical treatments, including bone regeneration. Regenerative procedures using a bioabsorbable collagen membrane (BCM) are well established in periodontal and implant dentistry. We evaluated the subsequent effects of the BCM in combination with OP-1 on bone regeneration in a rat mandibular circular critical-sized bone defect in vivo. Design We used 8 rats that received surgery in both sides of the mandible, and created the total 16 defects which were divided into 4 groups: Group 1; no treatment, as a control, Group 2; BCM alone, Group 3; BCM containing low dose 0.5 μg of OP-1 (L-OP-1), and Group 4; BCM containing high dose 2.0 μg of OP-1 (H-OP-1). Newly formed bone was evaluated by micro computed tomography (micro-CT) and histological analyses at 8 weeks postoperatively. In quantitative and qualitative micro-CT analyses of the volume of new bone formation, bone density, and percentage of new bone area was evaluated. Results BCM with rhOP-1 significantly increased and accelerated bone volume, bone mineral density, and percentage of new bone area compared to control and BCM alone at 8 weeks after surgery; these enhancements in bone regeneration in the OP-1-treated groups were dose-dependent. Conclusions OP-1 delivered with a BCM may have effective osteoinductive potency and be a good combination for bone regeneration. The use of such a combination device for osteogenesis may result in safer and more predictable bone regenerative outcomes in the future.
KW - Bone regeneration
KW - Collagen membrane
KW - Micro-CT analysis
KW - Osteogenesis
KW - Osteogenic protein-1
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U2 - 10.1016/j.archoralbio.2017.09.005
DO - 10.1016/j.archoralbio.2017.09.005
M3 - Article
C2 - 28938197
AN - SCOPUS:85029489696
VL - 84
SP - 19
EP - 28
JO - Archives of Oral Biology
JF - Archives of Oral Biology
SN - 0003-9969
ER -