A cosmid vector containing a dominant selectable marker for cloning Chlamydomonas genes by complementation

Mary Chang, Fei Li, O. W. Odom, Jaesung Lee, David L. Herrin

Research output: Contribution to journalArticle

Abstract

Cosmid vectors containing a dominate selectable marker (ble) for complementation cloning of genes in Chlamydomonas reinhardtii were created. The usefulness of these vectors, which differ in the orientation of the ble cassette, was demonstrated by transforming C. reinhardtii to phleomycin resistance, by constructing a large library (∼5×105 recombinants) in one of them using DNA from a C. reinhardtii mutant, and by transforming C. reinhardtii with recombinant cosmid clones and pools.

Original languageEnglish (US)
Pages (from-to)75-78
Number of pages4
JournalPlasmid
Volume49
Issue number1
DOIs
StatePublished - Jan 2003

Keywords

  • Chlamydomonas
  • Complementation cloning
  • Cosmid vector
  • Dominant marker
  • Phleomycin resistance
  • ble cassette

ASJC Scopus subject areas

  • Molecular Biology

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