A cosmid vector containing a dominant selectable marker for cloning Chlamydomonas genes by complementation

Mary Chang; Fei Li; O. W. Odom; Jaesung Lee; David L. Herrin

doi:10.1016/S0147-619X(02)00158-0

A cosmid vector containing a dominant selectable marker for cloning Chlamydomonas genes by complementation

Mary Chang, Fei Li, O. W. Odom, Jaesung Lee, David L. Herrin

Biology and Genomics

Research output: Contribution to journal › Article › peer-review

Abstract

Cosmid vectors containing a dominate selectable marker (ble) for complementation cloning of genes in Chlamydomonas reinhardtii were created. The usefulness of these vectors, which differ in the orientation of the ble cassette, was demonstrated by transforming C. reinhardtii to phleomycin resistance, by constructing a large library (∼5×10⁵ recombinants) in one of them using DNA from a C. reinhardtii mutant, and by transforming C. reinhardtii with recombinant cosmid clones and pools.

Original language	English (US)
Pages (from-to)	75-78
Number of pages	4
Journal	Plasmid
Volume	49
Issue number	1
DOIs	https://doi.org/10.1016/S0147-619X(02)00158-0
State	Published - Jan 2003

Keywords

Chlamydomonas
Complementation cloning
Cosmid vector
Dominant marker
Phleomycin resistance
ble cassette

ASJC Scopus subject areas

Molecular Biology

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10.1016/S0147-619X(02)00158-0

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title = "A cosmid vector containing a dominant selectable marker for cloning Chlamydomonas genes by complementation",

abstract = "Cosmid vectors containing a dominate selectable marker (ble) for complementation cloning of genes in Chlamydomonas reinhardtii were created. The usefulness of these vectors, which differ in the orientation of the ble cassette, was demonstrated by transforming C. reinhardtii to phleomycin resistance, by constructing a large library (∼5×105 recombinants) in one of them using DNA from a C. reinhardtii mutant, and by transforming C. reinhardtii with recombinant cosmid clones and pools.",

keywords = "Chlamydomonas, Complementation cloning, Cosmid vector, Dominant marker, Phleomycin resistance, ble cassette",

author = "Mary Chang and Fei Li and Odom, {O. W.} and Jaesung Lee and Herrin, {David L.}",

note = "Funding Information: This research was supported by grants from the USDA (NRICGP 99-35301-7847) and the Robert A. Welch Foundation (F-1164). ",

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T1 - A cosmid vector containing a dominant selectable marker for cloning Chlamydomonas genes by complementation

AU - Chang, Mary

AU - Li, Fei

AU - Odom, O. W.

AU - Lee, Jaesung

AU - Herrin, David L.

N1 - Funding Information: This research was supported by grants from the USDA (NRICGP 99-35301-7847) and the Robert A. Welch Foundation (F-1164).

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Y1 - 2003/1

N2 - Cosmid vectors containing a dominate selectable marker (ble) for complementation cloning of genes in Chlamydomonas reinhardtii were created. The usefulness of these vectors, which differ in the orientation of the ble cassette, was demonstrated by transforming C. reinhardtii to phleomycin resistance, by constructing a large library (∼5×105 recombinants) in one of them using DNA from a C. reinhardtii mutant, and by transforming C. reinhardtii with recombinant cosmid clones and pools.

AB - Cosmid vectors containing a dominate selectable marker (ble) for complementation cloning of genes in Chlamydomonas reinhardtii were created. The usefulness of these vectors, which differ in the orientation of the ble cassette, was demonstrated by transforming C. reinhardtii to phleomycin resistance, by constructing a large library (∼5×105 recombinants) in one of them using DNA from a C. reinhardtii mutant, and by transforming C. reinhardtii with recombinant cosmid clones and pools.

KW - Chlamydomonas

KW - Complementation cloning

KW - Cosmid vector

KW - Dominant marker

KW - Phleomycin resistance

KW - ble cassette

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A cosmid vector containing a dominant selectable marker for cloning Chlamydomonas genes by complementation

Abstract

Keywords

ASJC Scopus subject areas

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