A disposable bio-nano-chip using agarose beads for high performance immunoassays

Nan Du, Jie Chou, Eliona Kulla, Pierre N. Floriano, Nicolaos Christodoulides, John T. McDevitt

Research output: Contribution to journalArticle

Abstract

This article reports on the fabrication of a disposable bio-nano-chip (BNC), a microfluidic device composed of polydimethylsiloxane (PDMS) and thiolene-based optical epoxy which is both cost-effective and suitable for high performance immunoassays. A novel room temperature (RT) bonding technique was utilized so as to achieve irreversible covalent bonding between PDMS and thiolene-based epoxy layers, while at the same time being compatible with the insertion of agarose bead sensors, selectively arranged in an array of pyramidal microcavities replicated in the thiolene thin film layer. In the sealed device, the bead-supporting epoxy film is sandwiched between two PDMS layers comprising of fluidic injection and drain channels. The agarose bead sensors used in the device are sensitized with anti-C-reactive protein (CRP) antibody, and a fluorescent sandwich-type immunoassay was run to characterize the performance of this device. Computational fluid dynamics (CFD) was used based on the device specifications to model the bead penetration. Experimental data revealed analyte penetration of the immunocomplex to 100. μm into the 280. μm diameter agarose beads, which correlated well with the simulation. A dose-response curve was obtained and the linear dynamic range of the assay was established over 1 ng/mL to 50 ng/mL with a limit of detection less than 1 ng/mL.

Original languageEnglish (US)
Pages (from-to)251-256
Number of pages6
JournalBiosensors and Bioelectronics
Volume28
Issue number1
DOIs
StatePublished - Oct 15 2011

Keywords

  • Agarose bead
  • CRP
  • Disposable
  • Immunoassay
  • Microfluidic
  • PDMS

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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