A disposable microfluidic point-of-care device for the detection of HIV: A new up-converting phosphor technology application

Z. Chen, P. L.A.M. Corstjens, M. Zuiderwijk, J. Wang, M. G. Mauk, H. H. Bau, W. R. Abrams, D. Malamud

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    A bench-top analysis protocol to detect HIV-1 antibodies in blood plasma with upconverting phosphor particles (UPT) and lateral flow (LF) was established. A preliminary clinical study demonstrated that the UPT-LF detection successfully identified positive and negative samples. A 104× dilution of a positive clinical sample generated a signal well above the negative control. The bench-top UPT-LF protocol was implemented in a microfluidic chip with functions such as sample introduction, buffer distribution, metering, mixing, and thermo -pneumatic pump ing. The flow control was achieved with phase-change (PC) valves that operated in a self-actuated mode. Preliminary tests of the microfluidic device demonstrated compatibility of the microfluidic chip with UPT-LF detection and produced comparable sensitivity to that of the bench-top test.

    Original languageEnglish (US)
    Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
    Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    PublisherTransducer Research Foundation
    Pages791-793
    Number of pages3
    ISBN (Print)0974361119, 9780974361116
    StatePublished - 2005
    Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
    Duration: Oct 9 2005Oct 13 2005

    Publication series

    NameMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    Volume1

    Other

    Other9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
    Country/TerritoryUnited States
    CityBoston, MA
    Period10/9/0510/13/05

    Keywords

    • HIV detection
    • Immunoassay
    • Lab-on-a-chip
    • Phase-change valve

    ASJC Scopus subject areas

    • Chemical Engineering (miscellaneous)
    • Bioengineering

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