A disposable microfluidic point-of-care device for the detection of HIV: A new up-converting phosphor technology application

Z. Chen, P. L.A.M. Corstjens, M. Zuiderwijk, J. Wang, M. G. Mauk, H. H. Bau, W. R. Abrams, D. Malamud

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A bench-top analysis protocol to detect HIV-1 antibodies in blood plasma with upconverting phosphor particles (UPT) and lateral flow (LF) was established. A preliminary clinical study demonstrated that the UPT-LF detection successfully identified positive and negative samples. A 104× dilution of a positive clinical sample generated a signal well above the negative control. The bench-top UPT-LF protocol was implemented in a microfluidic chip with functions such as sample introduction, buffer distribution, metering, mixing, and thermo -pneumatic pump ing. The flow control was achieved with phase-change (PC) valves that operated in a self-actuated mode. Preliminary tests of the microfluidic device demonstrated compatibility of the microfluidic chip with UPT-LF detection and produced comparable sensitivity to that of the bench-top test.

Original languageEnglish (US)
Title of host publicationMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference
Subtitle of host publication9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherTransducer Research Foundation
Pages791-793
Number of pages3
ISBN (Print)0974361119, 9780974361116
StatePublished - 2005
Event9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005 - Boston, MA, United States
Duration: Oct 9 2005Oct 13 2005

Publication series

NameMicro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Volume1

Other

Other9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005
CountryUnited States
CityBoston, MA
Period10/9/0510/13/05

Keywords

  • HIV detection
  • Immunoassay
  • Lab-on-a-chip
  • Phase-change valve

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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