A DNA microarray-based genetic screen for nonhomologous end-joining mutants in Saccharomyces cerevisiae

S. L. Ooi; D. D. Shoemaker; J. D. Boeke

doi:10.1126/science.1065672

A DNA microarray-based genetic screen for nonhomologous end-joining mutants in Saccharomyces cerevisiae

S. L. Ooi, D. D. Shoemaker, J. D. Boeke

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

We describe a microarray-based screen performed by imposing different genetic selections on thousands of yeast mutants in parallel, representing most genes in the yeast genome. The presence or absence of mutants was detected by oligonucleotide arrays that hybridize to 20-nucleotide "barcodes." We used this method to screen for components of the nonhomologous end-joining (NHEJ) pathway. Known components of the pathway were identified, as well as a gene not previously known to be involved in NHEJ, NEJ1. Nej1 protein interacts with the amino terminus of LIF1/XRCC4, a recently recognized "guardian of the genome" against cancer.

Original language	English (US)
Pages (from-to)	2552-2556
Number of pages	5
Journal	Science
Volume	294
Issue number	5551
DOIs	https://doi.org/10.1126/science.1065672
State	Published - Dec 21 2001

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A DNA microarray-based genetic screen for nonhomologous end-joining mutants in Saccharomyces cerevisiae

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