A fast full-body fluorescence/bioluminescence imaging system for small animals

Jong Hwan Lee, Hyun Keol Kim, Jingfei Jia, Christopher Fong, Andreas H. Hielscher

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    Whole body in vivo optical imaging of small animals has widened its applications and increased the capabilities for preclinical researches. However, most commercial and prototype optical imaging systems are camera-based systems using epi- or trans- illumination mode, with limited views of small animals. And for more accurate tomographic image reconstruction, additional data and information of a target animal is necessary. To overcome these issues, researchers have suggested several approaches such as maximizing the detection area or using the information of other highresolution modalities such as CT, MRI or Ultrasound, or using multi-spectral signals. As one of ways to maximizing the detection area of a target animal, we present a new fluorescence and bioluminescence imaging system for small animals, which can image entire surface of a target animal simultaneously. This system uses double mirror reflection scheme and it consists of input unit, imaging unit with two conical mirrors, the source illumination part and the surface scanner, and the detection unit with an intensified CCD camera system. Two conical mirrors are configured that a larger size mirror captures a target animal surface, and a smaller size mirror projects this captured image onto a CCD camera with one acquisition. With this scheme, we could capture entire surface of a target animal simultaneously and improve back reflection issue between a mirror and an animal surface of a single conical mirror scheme. Additionally, we could increase accessibility to an animal for multi-modality integration by providing unobstructed space around a target animal.

    Original languageEnglish (US)
    Title of host publicationOptical Tomography and Spectroscopy of Tissue X
    DOIs
    StatePublished - 2013
    EventOptical Tomography and Spectroscopy of Tissue X - San Francisco, CA, United States
    Duration: Feb 3 2013Feb 6 2013

    Publication series

    NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
    Volume8578
    ISSN (Print)1605-7422

    Conference

    ConferenceOptical Tomography and Spectroscopy of Tissue X
    CountryUnited States
    CitySan Francisco, CA
    Period2/3/132/6/13

    Keywords

    • bioluminescence tomography
    • fluorescence molecular tomography
    • instrumentation
    • Optical tomography
    • small animal imaging

    ASJC Scopus subject areas

    • Electronic, Optical and Magnetic Materials
    • Biomaterials
    • Atomic and Molecular Physics, and Optics
    • Radiology Nuclear Medicine and imaging

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