A genomewide search for ribozymes reveals an HDV-like sequence in the human CPEB3 gene

Kourosh Salehi-Ashtiani, Andrej Lupták, Alexander Litovchick, Jack W. Szostak

Research output: Contribution to journalArticle

Abstract

Ribozymes are thought to have played a pivotal role in the early evolution of life, but relatively few have been identified in modern organisms. We performed an in vitro selection aimed at isolating self-cleaving RNAs from the human genome. The selection yielded several ribozymes, one of which is a conserved mammalian sequence that resides in an intron of the CPEB3 gene, which belongs to a family of genes regulating messenger RNA polyadenylation. The CPEB3 ribozyme is structurally and biochemically related to the human hepatitis delta virus (HDV) ribozymes. The occurrence of this ribozyme exclusively in mammals suggests that it may have evolved as recently as 200 million years ago. We postulate that HDV arose from the human transcriptome.

Original languageEnglish (US)
Pages (from-to)1788-1792
Number of pages5
JournalScience
Volume313
Issue number5794
DOIs
StatePublished - Sep 22 2006

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