A green fluorescent protein enhancer trap screen in Drosophila photoreceptor cells

Bertrand Mollereau, Mathias F. Wernet, Philippe Beaufils, Darrell Killian, Franck Pichaud, Ronald Kühnlein, Claude Desplan

Research output: Contribution to journalArticlepeer-review

Abstract

The Drosophila ommatidia contain two classes of photoreceptor cells (PR's), the outer and the inner PR's. We performed an enhancer trap screen in order to target genes specifically expressed in PR's. Using the UAS/GAL4 method with enhanced green fluorescent protein (eGFP) as a vital marker, we screened 180 000 flies. Out of 2730 lines exhibiting new eGFP patterns, we focused on 16 lines expressing eGFP in particular subsets of PR's. In particular, we describe three lines inserted near the spalt major, m-spondin and furrowed genes, whose respective expression patterns resemble those genes. These genes had not been reported to be expressed in the adult eye. These examples clearly show the ability of our screen to target genes expressed in the adult Drosophila eye. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish (US)
Pages (from-to)151-160
Number of pages10
JournalMechanisms of Development
Volume93
Issue number1-2
DOIs
StatePublished - May 1 2000

Keywords

  • Antenna
  • Bristle
  • Drosophila photoreceptors
  • Enhanced green fluorescent protein
  • Enhancer trap
  • Furrowed
  • GAL4
  • Labellum
  • Lamina
  • Leg
  • Maxillary palpus
  • Medulla
  • Ocelli
  • Optic lobe
  • R1-6 cells
  • R7 cells
  • R8 cells
  • Rhodopsin
  • Scutellum
  • Spalt
  • Wing
  • m-spondin

ASJC Scopus subject areas

  • Embryology
  • Developmental Biology

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