TY - GEN
T1 - A herringbone microfluidic probe for affinity separation of cells
AU - Glia, Ayoub
AU - Sukumar, Pavithra
AU - Deliorman, Muhammedin
AU - Qasaimeh, Mohammad A.
N1 - Funding Information:
Ministry of Education (Taiwan) (“Higher Education Sprout Project” under a grant number of 108Q2713E1).
Funding Information:
The authors would like to thank 1) Ministry of Science and Technology (MOST) of Taiwan for funding this work (MOST 107-2314-B-007-005), 2) National Health Research Institutes (NHRI-EX108-10828EI), and 3)
Funding Information:
We acknowledge funding from the NYUAD Research Enhancement Fund (REF2017), NYU Abu Dhabi, UAE, the Sheikh Hamdan Medical Research Grant Award, Dubai, UAE, and the Terry Fox Foundation's International Run Program, Vancouver, Canada. A.G. acknowledges the NYUAD Global PhD Fellowship.
Publisher Copyright:
© 2019 CBMS-0001.
PY - 2019
Y1 - 2019
N2 - This work introduces a new concept of capturing cells on functionalized substrates within an open microfluidic system. The capture substrate is physically decoupled from the microfluidic delivery system and thus it can be independently functionalized with multiple recognition biomolecules such as antibodies and aptamers. Further, captured cells can be characterized without releasing them from the substrate (e.g. nano-mechanical phenotyping using atomic force microscopy), can be locally tested with drugs, and can be selectively lysed and analyzed. Hence, the developed tool opens new venues for researchers with interest in the study of circulating tumor cells.
AB - This work introduces a new concept of capturing cells on functionalized substrates within an open microfluidic system. The capture substrate is physically decoupled from the microfluidic delivery system and thus it can be independently functionalized with multiple recognition biomolecules such as antibodies and aptamers. Further, captured cells can be characterized without releasing them from the substrate (e.g. nano-mechanical phenotyping using atomic force microscopy), can be locally tested with drugs, and can be selectively lysed and analyzed. Hence, the developed tool opens new venues for researchers with interest in the study of circulating tumor cells.
KW - Circulating Tumor Cells
KW - Herringbone Micromixers
KW - Immuno-Capture
KW - Microfluidic Probe
UR - http://www.scopus.com/inward/record.url?scp=85094943448&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85094943448&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85094943448
T3 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
SP - 630
EP - 631
BT - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
PB - Chemical and Biological Microsystems Society
T2 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Y2 - 27 October 2019 through 31 October 2019
ER -