A Map of the Interactome Network of the Metazoan C. elegans

Siming Li, Christopher M. Armstrong, Nicolas Bertin, Hui Ge, Stuart Milstein, Mike Boxem, Pierre Olivier Vidalain, Jing Dong J Han, Alban Chesneau, Tong Hao, Debra S. Goldberg, Ning Li, Monica Martinez, Jean François Rual, Philippe Lamesch, Lai Xu, Muneesh Tewari, Sharyl L. Wong, Lan V. Zhang, Gabriel F. BerrizLaurent Jacotot, Philippe Vaglio, Jérôme Reboul, Tomoko Hirozane-Kishikawa, Qianru Li, Harrison W. Gabel, Ahmed Elewa, Bridget Baumgartner, Debra J. Rose, Haiyuan Yu, Stephanie Bosak, Reynaldo Sequerra, Andrew Fraser, Susan E. Mango, William M. Saxton, Susan Strome, Sander v. Van Den Heuvel, Fabio Piano, Jean Vandenhaute, Claude Sardet, Mark Gerstein, Lynn Doucette-Stamm, Kristin C. Gunsalus, J. Wade Harper, Michael E. Cusick, Frederick P. Roth, David E. Hill, Marc Vidal

Research output: Contribution to journalArticle

Abstract

To initiate studies on how protein-protein interaction (or "interactome") networks relate to multicellular functions, we have mapped a large fraction of the Caenorhabditis elegans interactome network. Starting with a subset of metazoan-specific proteins, more than 4000 interactions were identified from high-throughput, yeast two-hybrid (HT=Y2H) screens. Independent coaffinity purification assays experimentally validated the overall quality of this Y2H data set. Together with already described Y2H interactions and interologs predicted in silico, the current version of the Worm Interactome (WI5) map contains ∼5500 interactions. Topological and biological features of this interactome network, as well as its integration with phenome and transcriptome data sets, lead to numerous biological hypotheses.

Original languageEnglish (US)
Pages (from-to)540-543
Number of pages4
JournalScience
Volume303
Issue number5657
DOIs
StatePublished - Jan 23 2004

ASJC Scopus subject areas

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    Li, S., Armstrong, C. M., Bertin, N., Ge, H., Milstein, S., Boxem, M., Vidalain, P. O., Han, J. D. J., Chesneau, A., Hao, T., Goldberg, D. S., Li, N., Martinez, M., Rual, J. F., Lamesch, P., Xu, L., Tewari, M., Wong, S. L., Zhang, L. V., ... Vidal, M. (2004). A Map of the Interactome Network of the Metazoan C. elegans. Science, 303(5657), 540-543. https://doi.org/10.1126/science.1091403