A mass-action-based model for gene expression regulation in dynamic systems

Guoshou Teo, Christine Vogel, Debashis Ghosh, Sinae Kim, Hyungwon Choi

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Although joint analysis of multiple omics data sets is often discussed in the context of analyzing large-scale genomic data in clinical or population studies, data integration is also useful for systems biology studies that investigate biological mechanisms in model systems under controlled environment. In this chapter, a model-based method is developed to simultaneously analyze time course transcriptomic and proteomic data sets to quantitatively dissect the contribution of RNA-level and protein-level regulation to the variation in gene expression. The statistical method is based on a mass-actionbased model for protein synthesis and degradation rates of individual genes, and change points in the stochastic process of the kinetic parameters are derived to identify distinct patterns of regulation of gene expression in time course profiles. A sampling-based inference procedure using Markov chainMonte Carlo is implemented, and the posterior probabilities of change points in the ratio of protein synthesis and degradation are used to control the Bayesian false discovery rate. The method is illustrated using a yeast data set monitoring mRNA and protein expression in hyperosmolarity shock, where stress response functions are immediately invoked by up-regulation at the mRNA and protein levels and translational machinery is shut down in the early time points but reactivated later in time points at the protein levels.

Original languageEnglish (US)
Title of host publicationIntegrating Omics Data
PublisherCambridge University Press
Pages362-379
Number of pages18
ISBN (Electronic)9781107706484
ISBN (Print)9781107069114
DOIs
StatePublished - Jan 1 2015

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint Dive into the research topics of 'A mass-action-based model for gene expression regulation in dynamic systems'. Together they form a unique fingerprint.

Cite this