Abstract
Systematic mappings of protein interactome networks have provided invaluable functional information for numerous model organisms. Here we develop PCR-mediated Linkage of barcoded Adapters To nucleic acid Elements for sequencing (PLATE-seq) that serves as a general tool to rapidly sequence thousands of DNA elements. We validate its utility by generating the ORFeome for Oryza sativa covering 2,300 genes and constructing a high-quality protein-protein interactome map consisting of 322 interactions between 289 proteins, expanding the known interactions in rice by roughly 50%. Our work paves the way for high-throughput profiling of protein-protein interactions in a wide range of organisms.
Original language | English (US) |
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Article number | 11836 |
Number of pages | 7 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Volume | 117 |
Issue number | 21 |
DOIs | |
State | Published - May 26 2020 |
Keywords
- Networks
- Next-generation sequencing
- ORFeome
- Protein-protein interaction
- Rice
- Protein Interaction Mapping/methods
- Open Reading Frames/genetics
- Oryza/genetics
- Databases, Genetic
- DNA, Plant/genetics
- Computational Biology/methods
- Genome, Plant/genetics
- Sequence Analysis, DNA/methods
- High-Throughput Nucleotide Sequencing/methods
- Protein Interaction Maps/genetics
ASJC Scopus subject areas
- General