A method for isolating and patch-clamping single mammalian taste receptor cells

Andrew I. Spielman, Istvan Mody, Joseph G. Brand, Glayde Whitney, John F. MacDonald, Michael W. Salter

Research output: Contribution to journalArticle

Abstract

Individual taste receptor cells were isolated from the tonque of the mouse by enzymatic treatment followed by mechanical dissociation. The cells were morphologically identical with taste cells from amphibians. Whole-cell voltage-clamp recordings indicated that the murine taste cells possess a variety of voltage-dependent inward and outward currents. Delayed rectifier currents were blocked by denatonium benzoate, one of the most bitter compounds known. This preparation should permit a detailed electrophysiological investigation of taste transduction in mammals at the level of taste receptor cells.

Original languageEnglish (US)
Pages (from-to)326-329
Number of pages4
JournalBrain Research
Volume503
Issue number2
DOIs
StatePublished - Dec 4 1989

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Keywords

  • Bitter
  • Denatonium benzoate
  • Taste cell
  • Whole-cell voltage-clamp

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Cite this

Spielman, A. I., Mody, I., Brand, J. G., Whitney, G., MacDonald, J. F., & Salter, M. W. (1989). A method for isolating and patch-clamping single mammalian taste receptor cells. Brain Research, 503(2), 326-329. https://doi.org/10.1016/0006-8993(89)91684-3