Abstract
Individual taste receptor cells were isolated from the tonque of the mouse by enzymatic treatment followed by mechanical dissociation. The cells were morphologically identical with taste cells from amphibians. Whole-cell voltage-clamp recordings indicated that the murine taste cells possess a variety of voltage-dependent inward and outward currents. Delayed rectifier currents were blocked by denatonium benzoate, one of the most bitter compounds known. This preparation should permit a detailed electrophysiological investigation of taste transduction in mammals at the level of taste receptor cells.
Original language | English (US) |
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Pages (from-to) | 326-329 |
Number of pages | 4 |
Journal | Brain Research |
Volume | 503 |
Issue number | 2 |
DOIs | |
State | Published - Dec 4 1989 |
Keywords
- Bitter
- Denatonium benzoate
- Taste cell
- Whole-cell voltage-clamp
ASJC Scopus subject areas
- General Neuroscience
- Molecular Biology
- Clinical Neurology
- Developmental Biology