TY - JOUR
T1 - A method for parental RNA interference in the wasp Nasonia vitripennis
AU - Lynch, Jeremy A.
AU - Desplan, Claude
N1 - Funding Information:
ACKNOWLEDGMENTS? ? We? would? like? to? thank? Mary? Anne? Pultz? and? David? Leaf? for? providing? invaluable? knowledge? and? assistance? in? working? with? Nasonia.? This? work? was? supported? by? a? NIH? grant? to? C.D.;? J.A.L.? was? supported? by? a? NIH? Training? Grant? and? a? Dean? Dissertation? Fellowship? from? NYU.? This? investigation? was? conducted? in? a? facility? constructed? with? support? from? a? Research? Facilities? Improvement? grant? from? NCRR,? NIH.
PY - 2006/6
Y1 - 2006/6
N2 - The wasp Nasonia vitripennis is emerging as a useful model organism in which to address a variety of biological questions, due, in part, to its ease of laboratory use, unique aspects of its biology and the sequencing of its genome. In order to take full advantage of the potential of this organism, methods for manipulating gene function are needed. To this end, a protocol for parental RNA interference (pRNAi) in N. vitripennis is described. pRNAi entails injecting pupae with double-stranded RNA, allowing the injected wasps to eclose and examining the progeny for developmental defects. This basic protocol is described in the context of the life cycle of N. vitripennis. This technique has been useful in elucidating the function of most, although not all, genes tested to date, and has potential applications beyond embryonic patterning. pRNAi experiments in Nasonia can be completed in as little as 2 weeks.
AB - The wasp Nasonia vitripennis is emerging as a useful model organism in which to address a variety of biological questions, due, in part, to its ease of laboratory use, unique aspects of its biology and the sequencing of its genome. In order to take full advantage of the potential of this organism, methods for manipulating gene function are needed. To this end, a protocol for parental RNA interference (pRNAi) in N. vitripennis is described. pRNAi entails injecting pupae with double-stranded RNA, allowing the injected wasps to eclose and examining the progeny for developmental defects. This basic protocol is described in the context of the life cycle of N. vitripennis. This technique has been useful in elucidating the function of most, although not all, genes tested to date, and has potential applications beyond embryonic patterning. pRNAi experiments in Nasonia can be completed in as little as 2 weeks.
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U2 - 10.1038/nprot.2006.70
DO - 10.1038/nprot.2006.70
M3 - Article
C2 - 17406271
AN - SCOPUS:33751067329
SN - 1754-2189
VL - 1
SP - 486
EP - 494
JO - Nature Protocols
JF - Nature Protocols
IS - 1
ER -