A method for rapid gain-of-function studies in the mouse embryonic nervous system

Nicholas Gaiano; Jhumku D. Kohtz; Daniel H. Turnbull; Gord Fishell

doi:10.1038/12186

A method for rapid gain-of-function studies in the mouse embryonic nervous system

Nicholas Gaiano, Jhumku D. Kohtz, Daniel H. Turnbull, Gord Fishell

Center for Genomics and Systems Biology

Research output: Contribution to journal › Article › peer-review

Abstract

We used ultrasound image-guided injections of high-titer retroviral vectors to obtain widespread introduction of genes into the mouse nervous system in utero as early as embryonic day 8.5 (E8.5). The vectors used included internal promoters that substantially improved proviral gene expression in the ventricular zone of the brain. To demonstrate the utility of this system, we extended our previous work in vitro by infecting the telencephalon in vivo as early as E8.5 with a virus expressing Sonic Hedgehog. Infected embryos showed gross morphological brain defects, as well as ectopic expression of ventral telencephalic markers characteristic of either the medial or lateral ganglionic eminences.

Original language	English (US)
Pages (from-to)	812-819
Number of pages	8
Journal	Nature Neuroscience
Volume	2
Issue number	9
DOIs	https://doi.org/10.1038/12186
State	Published - Sep 1999

ASJC Scopus subject areas

Neuroscience(all)

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10.1038/12186

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@article{a8dbb555b7af447290b959d709a6f9f6,

title = "A method for rapid gain-of-function studies in the mouse embryonic nervous system",

abstract = "We used ultrasound image-guided injections of high-titer retroviral vectors to obtain widespread introduction of genes into the mouse nervous system in utero as early as embryonic day 8.5 (E8.5). The vectors used included internal promoters that substantially improved proviral gene expression in the ventricular zone of the brain. To demonstrate the utility of this system, we extended our previous work in vitro by infecting the telencephalon in vivo as early as E8.5 with a virus expressing Sonic Hedgehog. Infected embryos showed gross morphological brain defects, as well as ectopic expression of ventral telencephalic markers characteristic of either the medial or lateral ganglionic eminences.",

author = "Nicholas Gaiano and Kohtz, {Jhumku D.} and Turnbull, {Daniel H.} and Gord Fishell",

note = "Funding Information: We thank Rusty Lansford for bringing the CA regulatory element to our attention and Alex Langston and Robin Kimmel for input at the start of this work. We also thank Maria McCarthy and Connie Cepko for discussions, Michelle Starz-Gaiano for reading the manuscript, Ulf Eriksson for providing the anti-CRBP antibody and K. Mahon for the dlx2 in situ probe. This work was supported by NIH grants NS32993 (G.F.), NS38461 (D.H.T.) and HL62334 (D.H.T.). Additional support was provided by March of Dimes Grant 6-FY99-634 (G.F.). N.G. is supported by a postdoctoral fellowship from the American Cancer Society (PF4473).",

year = "1999",

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doi = "10.1038/12186",

language = "English (US)",

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A method for rapid gain-of-function studies in the mouse embryonic nervous system

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