A method for rapid gain-of-function studies in the mouse embryonic nervous system

Nicholas Gaiano, Jhumku D. Kohtz, Daniel H. Turnbull, Gord Fishell

Research output: Contribution to journalArticle

Abstract

We used ultrasound image-guided injections of high-titer retroviral vectors to obtain widespread introduction of genes into the mouse nervous system in utero as early as embryonic day 8.5 (E8.5). The vectors used included internal promoters that substantially improved proviral gene expression in the ventricular zone of the brain. To demonstrate the utility of this system, we extended our previous work in vitro by infecting the telencephalon in vivo as early as E8.5 with a virus expressing Sonic Hedgehog. Infected embryos showed gross morphological brain defects, as well as ectopic expression of ventral telencephalic markers characteristic of either the medial or lateral ganglionic eminences.

Original languageEnglish (US)
Pages (from-to)812-819
Number of pages8
JournalNature Neuroscience
Volume2
Issue number9
DOIs
StatePublished - Sep 1999

ASJC Scopus subject areas

  • Neuroscience(all)

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