A microfluidic culture system for screening of chemical defined conditions for human embryonic stem cells (hESCs)

Ken Ichiro Kamei; Shuling Guo; Minori Ohashi; Zeta Tak For Yu; Eric Gschweng; Jinghua Tang; Jami McLaughlin; Ki Bum Lee; Owen N. Witte; Hsian Rong Tseng

A microfluidic culture system for screening of chemical defined conditions for human embryonic stem cells (hESCs)

Ken Ichiro Kamei, Shuling Guo, Minori Ohashi, Zeta Tak For Yu, Eric Gschweng, Jinghua Tang, Jami McLaughlin, Ki Bum Lee, Owen N. Witte, Hsian Rong Tseng

Biology

Research output: Contribution to conference › Paper › peer-review

Abstract

We have successfully developed an integrated microfluidic platform, the hESC-μChip, to culture and analyze individual hESC colonies reproducibly, quantitatively and semi-automatedly. This hESC-μChip has six individually addressable culture chambers for performing multiparameteric analyses. On-chip immunoassays of pluripotency markers confirmed that the hESC colonies had maintained their pluripotency. It is also a demonstration of parallel/multiparameteric/real-time/automated examination of self-renewal and differentiation of hESCs in the same device.

Original language	English (US)
Pages	1974-1976
Number of pages	3
State	Published - 2008
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: Oct 12 2008 → Oct 16 2008

Other

Other	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	10/12/08 → 10/16/08

Keywords

Human embryonic stem cell
Microfluidics
Screening
Self-renewal

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

Kamei, K. I., Guo, S., Ohashi, M., Yu, Z. T. F., Gschweng, E., Tang, J., McLaughlin, J., Lee, K. B., Witte, O. N., & Tseng, H. R. (2008). A microfluidic culture system for screening of chemical defined conditions for human embryonic stem cells (hESCs). 1974-1976. Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

A microfluidic culture system for screening of chemical defined conditions for human embryonic stem cells (hESCs). / Kamei, Ken Ichiro; Guo, Shuling; Ohashi, Minori et al.
2008. 1974-1976 Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

Research output: Contribution to conference › Paper › peer-review

Kamei, KI, Guo, S, Ohashi, M, Yu, ZTF, Gschweng, E, Tang, J, McLaughlin, J, Lee, KB, Witte, ON & Tseng, HR 2008, 'A microfluidic culture system for screening of chemical defined conditions for human embryonic stem cells (hESCs)', Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States, 10/12/08 - 10/16/08 pp. 1974-1976.

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abstract = "We have successfully developed an integrated microfluidic platform, the hESC-μChip, to culture and analyze individual hESC colonies reproducibly, quantitatively and semi-automatedly. This hESC-μChip has six individually addressable culture chambers for performing multiparameteric analyses. On-chip immunoassays of pluripotency markers confirmed that the hESC colonies had maintained their pluripotency. It is also a demonstration of parallel/multiparameteric/real-time/automated examination of self-renewal and differentiation of hESCs in the same device.",

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author = "Kamei, {Ken Ichiro} and Shuling Guo and Minori Ohashi and Yu, {Zeta Tak For} and Eric Gschweng and Jinghua Tang and Jami McLaughlin and Lee, {Ki Bum} and Witte, {Owen N.} and Tseng, {Hsian Rong}",

year = "2008",

language = "English (US)",

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AU - Kamei, Ken Ichiro

AU - Guo, Shuling

AU - Ohashi, Minori

AU - Yu, Zeta Tak For

AU - Gschweng, Eric

AU - Tang, Jinghua

AU - McLaughlin, Jami

AU - Lee, Ki Bum

AU - Witte, Owen N.

AU - Tseng, Hsian Rong

PY - 2008

Y1 - 2008

N2 - We have successfully developed an integrated microfluidic platform, the hESC-μChip, to culture and analyze individual hESC colonies reproducibly, quantitatively and semi-automatedly. This hESC-μChip has six individually addressable culture chambers for performing multiparameteric analyses. On-chip immunoassays of pluripotency markers confirmed that the hESC colonies had maintained their pluripotency. It is also a demonstration of parallel/multiparameteric/real-time/automated examination of self-renewal and differentiation of hESCs in the same device.

AB - We have successfully developed an integrated microfluidic platform, the hESC-μChip, to culture and analyze individual hESC colonies reproducibly, quantitatively and semi-automatedly. This hESC-μChip has six individually addressable culture chambers for performing multiparameteric analyses. On-chip immunoassays of pluripotency markers confirmed that the hESC colonies had maintained their pluripotency. It is also a demonstration of parallel/multiparameteric/real-time/automated examination of self-renewal and differentiation of hESCs in the same device.

KW - Human embryonic stem cell

KW - Microfluidics

KW - Screening

KW - Self-renewal

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T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

A microfluidic culture system for screening of chemical defined conditions for human embryonic stem cells (hESCs)

Abstract

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Keywords

ASJC Scopus subject areas

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