A microfluidic system for saliva-based detection of infectious diseases

Zongyuan Chen, Michael G. Mauk, Jing Wang, William R. Abrams, Paul L.A.M. Corstjens, R. Sam Niedbala, Daniel Malamud, Haim H. Bau

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A "lab-on-a-chip" system for detecting bacterial pathogens in oral fluid samples is described. The system comprises : (1) an oral fluid sample collector; (2) a disposable, plastic microfluidic cassette ("chip") for sample processing including immunochromatographic assay with a nitrocellulose lateral flow strip; (3) a platform that controls the cassette operation by providing metered quantities of reagents, temperature regulation, valve actuation; and (4) a laser scanner to interrogate the lateral flow strip. The microfluidic chip hosts a fluidic network for cell lysis, nucleic acid extraction and isolation, PCR, and labeling of the PCR product with bioconjugated, upconverting phosphor particles for detection on the lateral flow strip.

Original languageEnglish (US)
Title of host publicationOral-based Diagnostics
PublisherBlackwell Publishing Inc.
Pages429-436
Number of pages8
ISBN (Print)157331661X, 9781573316613
DOIs
StatePublished - Mar 2007

Publication series

NameAnnals of the New York Academy of Sciences
Volume1098
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Keywords

  • Diagnostics
  • Immunochromatography
  • Infectious diseases
  • Lab-on-a-chip
  • Microfluidics

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

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