A microfluidic system for saliva-based detection of infectious diseases

Zongyuan Chen, Michael G. Mauk, Jing Wang, William R. Abrams, Paul L.A.M. Corstjens, R. Sam Niedbala, Daniel Malamud, Haim H. Bau

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    A "lab-on-a-chip" system for detecting bacterial pathogens in oral fluid samples is described. The system comprises : (1) an oral fluid sample collector; (2) a disposable, plastic microfluidic cassette ("chip") for sample processing including immunochromatographic assay with a nitrocellulose lateral flow strip; (3) a platform that controls the cassette operation by providing metered quantities of reagents, temperature regulation, valve actuation; and (4) a laser scanner to interrogate the lateral flow strip. The microfluidic chip hosts a fluidic network for cell lysis, nucleic acid extraction and isolation, PCR, and labeling of the PCR product with bioconjugated, upconverting phosphor particles for detection on the lateral flow strip.

    Original languageEnglish (US)
    Title of host publicationOral-based Diagnostics
    PublisherBlackwell Publishing Inc.
    Pages429-436
    Number of pages8
    ISBN (Print)157331661X, 9781573316613
    DOIs
    StatePublished - Mar 2007

    Publication series

    NameAnnals of the New York Academy of Sciences
    Volume1098
    ISSN (Print)0077-8923
    ISSN (Electronic)1749-6632

    Keywords

    • Diagnostics
    • Immunochromatography
    • Infectious diseases
    • Lab-on-a-chip
    • Microfluidics

    ASJC Scopus subject areas

    • General Neuroscience
    • General Biochemistry, Genetics and Molecular Biology
    • History and Philosophy of Science

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