A new criterion for implicating Clostridium perfringens as the cause of food poisoning

V. R. Dowell; M. J. Torres-Anjel; H. P. Riemann; M. Merson; D. Whaley; G. Darland

A new criterion for implicating Clostridium perfringens as the cause of food poisoning

V. R. Dowell, M. J. Torres-Anjel, H. P. Riemann, M. Merson, D. Whaley, G. Darland

Global Public Health

Research output: Contribution to journal › Article › peer-review

Abstract

For the first time the enterotoxin of Clostridium perfringens type A (CPE) was demonstrated in stools from patients suffering from the specific type of 'food poisoning'. Sensitivity was 100% and specificity 94% vs. diarrhea reporting. The only person in which the CPE was demonstrated without his reporting diarrhea, had consumed implicated food; and the suspect strain, Hobb's type 1, was isolated from him. The samples were studied in a 'blind' fashion, during an outbreak epidemiologically and bacteriologically assigned to C. perfringens. The methodology was based on reversed, passive, hemagglutination. Glutaraldehyde was both the fixative as well as Y globulin sensitizer (from anti CPE rabbit serum), upon sheep red blood cells. The simplicity of the test will allow for detection of CPE in stools from patients at local public health laboratories of any capacity. Protocols to be followed in further outbreaks are recommended

Original language	English (US)
Pages (from-to)	137-142
Number of pages	6
Journal	Revista Latinoamericana de Microbiologia
Volume	17
Issue number	3
State	Published - 1975

ASJC Scopus subject areas

Microbiology
General Veterinary
Microbiology (medical)

Cite this

@article{b23e86ba0c2c4332acbb06dcc92472a2,

title = "A new criterion for implicating Clostridium perfringens as the cause of food poisoning",

abstract = "For the first time the enterotoxin of Clostridium perfringens type A (CPE) was demonstrated in stools from patients suffering from the specific type of 'food poisoning'. Sensitivity was 100% and specificity 94% vs. diarrhea reporting. The only person in which the CPE was demonstrated without his reporting diarrhea, had consumed implicated food; and the suspect strain, Hobb's type 1, was isolated from him. The samples were studied in a 'blind' fashion, during an outbreak epidemiologically and bacteriologically assigned to C. perfringens. The methodology was based on reversed, passive, hemagglutination. Glutaraldehyde was both the fixative as well as Y globulin sensitizer (from anti CPE rabbit serum), upon sheep red blood cells. The simplicity of the test will allow for detection of CPE in stools from patients at local public health laboratories of any capacity. Protocols to be followed in further outbreaks are recommended",

author = "Dowell, {V. R.} and Torres-Anjel, {M. J.} and Riemann, {H. P.} and M. Merson and D. Whaley and G. Darland",

year = "1975",

language = "English (US)",

volume = "17",

pages = "137--142",

journal = "Revista Latinoamericana de Microbiologia",

issn = "0187-4640",

publisher = "Mexico Asociacion Mexicana De Microbiologia",

number = "3",

}

TY - JOUR

T1 - A new criterion for implicating Clostridium perfringens as the cause of food poisoning

AU - Dowell, V. R.

AU - Torres-Anjel, M. J.

AU - Riemann, H. P.

AU - Merson, M.

AU - Whaley, D.

AU - Darland, G.

PY - 1975

Y1 - 1975

N2 - For the first time the enterotoxin of Clostridium perfringens type A (CPE) was demonstrated in stools from patients suffering from the specific type of 'food poisoning'. Sensitivity was 100% and specificity 94% vs. diarrhea reporting. The only person in which the CPE was demonstrated without his reporting diarrhea, had consumed implicated food; and the suspect strain, Hobb's type 1, was isolated from him. The samples were studied in a 'blind' fashion, during an outbreak epidemiologically and bacteriologically assigned to C. perfringens. The methodology was based on reversed, passive, hemagglutination. Glutaraldehyde was both the fixative as well as Y globulin sensitizer (from anti CPE rabbit serum), upon sheep red blood cells. The simplicity of the test will allow for detection of CPE in stools from patients at local public health laboratories of any capacity. Protocols to be followed in further outbreaks are recommended

AB - For the first time the enterotoxin of Clostridium perfringens type A (CPE) was demonstrated in stools from patients suffering from the specific type of 'food poisoning'. Sensitivity was 100% and specificity 94% vs. diarrhea reporting. The only person in which the CPE was demonstrated without his reporting diarrhea, had consumed implicated food; and the suspect strain, Hobb's type 1, was isolated from him. The samples were studied in a 'blind' fashion, during an outbreak epidemiologically and bacteriologically assigned to C. perfringens. The methodology was based on reversed, passive, hemagglutination. Glutaraldehyde was both the fixative as well as Y globulin sensitizer (from anti CPE rabbit serum), upon sheep red blood cells. The simplicity of the test will allow for detection of CPE in stools from patients at local public health laboratories of any capacity. Protocols to be followed in further outbreaks are recommended

UR - http://www.scopus.com/inward/record.url?scp=0016703158&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016703158&partnerID=8YFLogxK

M3 - Article

C2 - 171713

AN - SCOPUS:0016703158

SN - 0187-4640

VL - 17

SP - 137

EP - 142

JO - Revista Latinoamericana de Microbiologia

JF - Revista Latinoamericana de Microbiologia

IS - 3

ER -

A new criterion for implicating Clostridium perfringens as the cause of food poisoning

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this