A new visualization approach for identifying mutations that affect differentiation and organization of the Drosophila ommatidia

F. Pichaud, C. Desplan

Research output: Contribution to journalArticle

Abstract

The Drosophila eye is widely used as a model system to study neuronal differentiation, survival and axon projection. Photoreceptor differentiation starts with the specification of a founder cell R8, which sequentially recruits other photoreceptor neurons to the ommatidium. The eight photoreceptors that compose each ommatidium exist in two chiral forms organized along two axes of symmetry and this pattern represents a paradigm to study tissue polarity. We have developed a method of fluoroscopy to visualize the different types of photoreceptors and the organization of the ommatidia in living animals. This allowed us to perform an F1 genetic screen to isolate mutants affecting photoreceptor differentiation, survival or planar polarity. We illustrate the power of this detection system using known genetic backgrounds and new mutations that affect ommatidia differentiation, morphology or chirality.

Original languageEnglish (US)
Pages (from-to)815-826
Number of pages12
JournalDevelopment
Volume128
Issue number6
StatePublished - 2001

Keywords

  • Drosophila eye
  • Genetic screen
  • Ommatidia
  • Planar polarity
  • Rhodopsin

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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