A novel method to isolate odontoblasts from rat incisor

L. Guo, J. E. Berry, M. J. Somerman, R. M. Davidson

Research output: Contribution to journalArticle

Abstract

Historically, odontoblasts have been isolated from rat incisor using a surgical curette to separate these cells from the dentin. Isolation of odontoblasts using this approach typically resulted in cells with membrane properties that made the application of patch-clamp electrophysiological techniques prohibitive. The studies here describe a new procedure for isolating mature odontoblasts from adult rat incisor to obtain enriched populations of intact, viable odontoblasts that can be readily studied using patch-clamp methodologies. Identification of isolated cells as odontoblasts was confirmed using in situ mRNA hybridization for expression of dentin sialoprotein, osteocalcin, bone sialoprotein, and type I collagen, and calcium flux was monitored in these cells by means of fura-2 microfluorometry. We suggest that either single odontoblasts or clusters of these cells isolated by this new method would be an ideal preparation for the study of odontoblast properties using electrophysiological techniques, in situ hybridization and/or microfluorometry.

Original languageEnglish (US)
Pages (from-to)212-216
Number of pages5
JournalCalcified Tissue International
Volume66
Issue number3
DOIs
StatePublished - 2000

Keywords

  • Bone sialoprotein
  • Dentin sialoprotein
  • Fura-2
  • Odontoblast
  • Osteocalcin

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine
  • Endocrinology

Fingerprint Dive into the research topics of 'A novel method to isolate odontoblasts from rat incisor'. Together they form a unique fingerprint.

  • Cite this