TY - JOUR
T1 - A nucleic acid probe labeled with desmethyl thiazole orange
T2 - A new type of hybridization-sensitive fluorescent oligonucleotide for live-cell RNA imaging
AU - Okamoto, Akimitsu
AU - Sugizaki, Kaori
AU - Yuki, Mizue
AU - Yanagisawa, Hiroyuki
AU - Ikeda, Shuji
AU - Sueoka, Takuma
AU - Hayashi, Gosuke
AU - Wang, Dan Ohtan
PY - 2013/1/14
Y1 - 2013/1/14
N2 - A new fluorescent nucleotide with desmethyl thiazole orange dyes, D′505, has been developed for expansion of the function of fluorescent probes for live-cell RNA imaging. The nucleoside unit of D′505 for DNA autosynthesis was soluble in organic solvents, which made the preparation of nucleoside units and the reactions in the cycles of DNA synthesis more efficient. The dyes of D′505-containing oligodeoxynucleotide were protonated below pH 7 and the oligodeoxynucleotide exhibited hybridization-sensitive fluorescence emission through the control of excitonic interactions of the dyes of D′505. The simplified procedure and effective hybridization-sensitive fluorescence emission produced multicolored hybridization-sensitive fluorescent probes, which were useful for live-cell RNA imaging. The acceptor-bleaching method gave us information on RNA in a specific cell among many living cells.
AB - A new fluorescent nucleotide with desmethyl thiazole orange dyes, D′505, has been developed for expansion of the function of fluorescent probes for live-cell RNA imaging. The nucleoside unit of D′505 for DNA autosynthesis was soluble in organic solvents, which made the preparation of nucleoside units and the reactions in the cycles of DNA synthesis more efficient. The dyes of D′505-containing oligodeoxynucleotide were protonated below pH 7 and the oligodeoxynucleotide exhibited hybridization-sensitive fluorescence emission through the control of excitonic interactions of the dyes of D′505. The simplified procedure and effective hybridization-sensitive fluorescence emission produced multicolored hybridization-sensitive fluorescent probes, which were useful for live-cell RNA imaging. The acceptor-bleaching method gave us information on RNA in a specific cell among many living cells.
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U2 - 10.1039/c2ob26707a
DO - 10.1039/c2ob26707a
M3 - Article
C2 - 23172393
AN - SCOPUS:84870895305
SN - 1477-0520
VL - 11
SP - 362
EP - 371
JO - Organic and Biomolecular Chemistry
JF - Organic and Biomolecular Chemistry
IS - 2
ER -