A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

Jef D. Boeke; Francois La Croute; Gerald R. Fink

doi:10.1007/BF00330984

A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

Jef D. Boeke, Francois La Croute, Gerald R. Fink

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

Abstract

Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection. Wild-type strains of yeast (or ura3 mutant strains containing a plasmid-borne URA3⁺ gene) are unable to grow on medium containing the pyrimidine analog 5-fluoro-orotic acid, whereas ura3^- mutants grow normally. This selection, based on the loss of orotidine-5′-phosphate decarboxylase activity seems applicable to a variety of eucaryotic and procaryotic cells.

Original language	English (US)
Pages (from-to)	345-346
Number of pages	2
Journal	MGG Molecular & General Genetics
Volume	197
Issue number	2
DOIs	https://doi.org/10.1007/BF00330984
State	Published - Nov 1984

ASJC Scopus subject areas

Genetics

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title = "A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance",

abstract = "Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection. Wild-type strains of yeast (or ura3 mutant strains containing a plasmid-borne URA3+ gene) are unable to grow on medium containing the pyrimidine analog 5-fluoro-orotic acid, whereas ura3- mutants grow normally. This selection, based on the loss of orotidine-5′-phosphate decarboxylase activity seems applicable to a variety of eucaryotic and procaryotic cells.",

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year = "1984",

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T1 - A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast

T2 - 5-fluoro-orotic acid resistance

AU - Boeke, Jef D.

AU - La Croute, Francois

AU - Fink, Gerald R.

PY - 1984/11

Y1 - 1984/11

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AB - Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection. Wild-type strains of yeast (or ura3 mutant strains containing a plasmid-borne URA3+ gene) are unable to grow on medium containing the pyrimidine analog 5-fluoro-orotic acid, whereas ura3- mutants grow normally. This selection, based on the loss of orotidine-5′-phosphate decarboxylase activity seems applicable to a variety of eucaryotic and procaryotic cells.

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A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

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