Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection. Wild-type strains of yeast (or ura3 mutant strains containing a plasmid-borne URA3+ gene) are unable to grow on medium containing the pyrimidine analog 5-fluoro-orotic acid, whereas ura3- mutants grow normally. This selection, based on the loss of orotidine-5′-phosphate decarboxylase activity seems applicable to a variety of eucaryotic and procaryotic cells.
|Original language||English (US)|
|Number of pages||2|
|Journal||MGG Molecular & General Genetics|
|State||Published - Nov 1984|
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