A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance

Jef D. Boeke, Francois La Croute, Gerald R. Fink

    Research output: Contribution to journalArticle

    Abstract

    Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection. Wild-type strains of yeast (or ura3 mutant strains containing a plasmid-borne URA3+ gene) are unable to grow on medium containing the pyrimidine analog 5-fluoro-orotic acid, whereas ura3- mutants grow normally. This selection, based on the loss of orotidine-5′-phosphate decarboxylase activity seems applicable to a variety of eucaryotic and procaryotic cells.

    Original languageEnglish (US)
    Pages (from-to)345-346
    Number of pages2
    JournalMGG Molecular & General Genetics
    Volume197
    Issue number2
    DOIs
    StatePublished - Nov 1984

    ASJC Scopus subject areas

    • Genetics

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