A Practical Step-by-Step Guide for Quantifying Retroactivity in Gene Networks

Research output: Chapter in Book/Report/Conference proceedingChapter


One of the fundamental properties of engineered large-scale complex systems is modularity. In synthetic biology, genetic parts exhibit context-dependent behavior. Here, we describe and quantify a major source of such behavior: retroactivity. In particular, we provide a step-by-step guide for characterizing retroactivity to restore the modular description of genetic modules. Additionally, we also discuss how retroactivity can be leveraged to quantify and maximize robustness to perturbations due to interconnection of genetic modules.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages19
StatePublished - 2021

Publication series

NameMethods in molecular biology (Clifton, N.J.)
PublisherHumana Press
ISSN (Print)1064-3745


  • Context-dependence
  • Gene transcription networks
  • Loading
  • Model order reduction
  • Modularity
  • Retroactivity
  • Synthetic biology
  • Gene Regulatory Networks
  • Synthetic Biology
  • Algorithms
  • Models, Biological
  • Computer Simulation
  • Transcription, Genetic
  • Systems Biology/methods

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology


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