@inbook{1c9b669bff0b4896a72531ed741f438b,
title = "A Practical Step-by-Step Guide for Quantifying Retroactivity in Gene Networks",
abstract = "One of the fundamental properties of engineered large-scale complex systems is modularity. In synthetic biology, genetic parts exhibit context-dependent behavior. Here, we describe and quantify a major source of such behavior: retroactivity. In particular, we provide a step-by-step guide for characterizing retroactivity to restore the modular description of genetic modules. Additionally, we also discuss how retroactivity can be leveraged to quantify and maximize robustness to perturbations due to interconnection of genetic modules.",
keywords = "Context-dependence, Gene transcription networks, Loading, Model order reduction, Modularity, Retroactivity, Synthetic biology, Gene Regulatory Networks, Synthetic Biology, Algorithms, Models, Biological, Computer Simulation, Transcription, Genetic, Systems Biology/methods",
author = "Andras Gyorgy",
note = "Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-1032-9_14",
language = "English (US)",
volume = "2229",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Humana Press Inc.",
pages = "293--311",
booktitle = "Methods in Molecular Biology",
}