TY - JOUR
T1 - A Protein Domain-Based Interactome Network for C. elegans Early Embryogenesis
AU - Boxem, Mike
AU - Maliga, Zoltan
AU - Klitgord, Niels
AU - Li, Na
AU - Lemmens, Irma
AU - Mana, Miyeko
AU - de Lichtervelde, Lorenzo
AU - Mul, Joram D.
AU - van de Peut, Diederik
AU - Devos, Maxime
AU - Simonis, Nicolas
AU - Yildirim, Muhammed A.
AU - Cokol, Murat
AU - Kao, Huey Ling
AU - de Smet, Anne Sophie
AU - Wang, Haidong
AU - Schlaitz, Anne Lore
AU - Hao, Tong
AU - Milstein, Stuart
AU - Fan, Changyu
AU - Tipsword, Mike
AU - Drew, Kevin
AU - Galli, Matilde
AU - Rhrissorrakrai, Kahn
AU - Drechsel, David
AU - Koller, Daphne
AU - Roth, Frederick P.
AU - Iakoucheva, Lilia M.
AU - Dunker, A. Keith
AU - Bonneau, Richard
AU - Gunsalus, Kristin C.
AU - Hill, David E.
AU - Piano, Fabio
AU - Tavernier, Jan
AU - van den Heuvel, Sander
AU - Hyman, Anthony A.
AU - Vidal, Marc
N1 - Funding Information:
We are grateful to X. Xin and C. Boone for sharing of the cDNA library and yeast strains, to Joe Hargitai for unparalleled parallel computing support, to IBM's World Community Grid ( http://www.wcgrid.org ), and to M. Cusick for critical reading of the manuscript. Support was provided by the Leukemia Research Foundation to M.B., the W.M. Keck foundation to M.V., the FWO-V to I.L., National Institutes of Health grants R21RR023114 (M.B. P.I.), R01HG001715 (M.V. P.I.), R33CA105405 (M.V. P.I.), R33CA81658 (M.V. P.I.), R21CA113711 (L.M.I. P.I.), U54 CA011295 (J. Nevins, PI; M.V. subcontract), and CA95281 (S.v.d.H.), United States Army Medical Research Acquisition Activity grant W23RYX-3275-N605 (K.C.G.), New York State Foundation of Science, Technology, and Academic Research grant C040066 (K.C.G.), National Science Foundation grants MCB 0444818 to L.M.I. and BDI-0345474 to D.K. and grants IUAP-P6:28, UG-GOA12051401, and FWO-G.0031.06 to J.T. M.V. is a “Chercheur Qualifié Honoraire” from the Fonds de la Recherche Scientifique (FRS-FNRS, French Community of Belgium).
PY - 2008/8/8
Y1 - 2008/8/8
N2 - Many protein-protein interactions are mediated through independently folding modular domains. Proteome-wide efforts to model protein-protein interaction or "interactome" networks have largely ignored this modular organization of proteins. We developed an experimental strategy to efficiently identify interaction domains and generated a domain-based interactome network for proteins involved in C. elegans early-embryonic cell divisions. Minimal interacting regions were identified for over 200 proteins, providing important information on their domain organization. Furthermore, our approach increased the sensitivity of the two-hybrid system, resulting in a more complete interactome network. This interactome modeling strategy revealed insights into C. elegans centrosome function and is applicable to other biological processes in this and other organisms.
AB - Many protein-protein interactions are mediated through independently folding modular domains. Proteome-wide efforts to model protein-protein interaction or "interactome" networks have largely ignored this modular organization of proteins. We developed an experimental strategy to efficiently identify interaction domains and generated a domain-based interactome network for proteins involved in C. elegans early-embryonic cell divisions. Minimal interacting regions were identified for over 200 proteins, providing important information on their domain organization. Furthermore, our approach increased the sensitivity of the two-hybrid system, resulting in a more complete interactome network. This interactome modeling strategy revealed insights into C. elegans centrosome function and is applicable to other biological processes in this and other organisms.
KW - PROTEINS
UR - http://www.scopus.com/inward/record.url?scp=48649096489&partnerID=8YFLogxK
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U2 - 10.1016/j.cell.2008.07.009
DO - 10.1016/j.cell.2008.07.009
M3 - Article
C2 - 18692475
AN - SCOPUS:48649096489
SN - 0092-8674
VL - 134
SP - 534
EP - 545
JO - Cell
JF - Cell
IS - 3
ER -