A protein-driven DNA device that measures the excess binding energy of proteins that distort DNA

Wanqiu Shen; Michael F. Bruist; Steven D. Goodman; Nadrian C. Seeman

doi:10.1002/anie.200460302

A protein-driven DNA device that measures the excess binding energy of proteins that distort DNA

Wanqiu Shen, Michael F. Bruist, Steven D. Goodman, Nadrian C. Seeman

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

A DNA nanomechanical device was constructed by linking two triple-helical DNA motifs with a shaft of conventional DNA bearing the binding site for the integration host factor (IHF, shown as green ellipse in the picture). Binding of IHF to this site causes marked shaft bending, which can be monitored by fluorescence resonance energy transfer between donor (D) and acceptor (A) dyes.

Original language	English (US)
Pages (from-to)	4750-4752
Number of pages	3
Journal	Angewandte Chemie - International Edition
Volume	43
Issue number	36
DOIs	https://doi.org/10.1002/anie.200460302
State	Published - Sep 4 2004

Keywords

DNA structures
Fluorescent probes
Molecular devices
Proteins

ASJC Scopus subject areas

Catalysis
Chemistry(all)

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10.1002/anie.200460302

Cite this

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abstract = "A DNA nanomechanical device was constructed by linking two triple-helical DNA motifs with a shaft of conventional DNA bearing the binding site for the integration host factor (IHF, shown as green ellipse in the picture). Binding of IHF to this site causes marked shaft bending, which can be monitored by fluorescence resonance energy transfer between donor (D) and acceptor (A) dyes.",

keywords = "DNA structures, Fluorescent probes, Molecular devices, Proteins",

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AU - Bruist, Michael F.

AU - Goodman, Steven D.

AU - Seeman, Nadrian C.

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AB - A DNA nanomechanical device was constructed by linking two triple-helical DNA motifs with a shaft of conventional DNA bearing the binding site for the integration host factor (IHF, shown as green ellipse in the picture). Binding of IHF to this site causes marked shaft bending, which can be monitored by fluorescence resonance energy transfer between donor (D) and acceptor (A) dyes.

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KW - Fluorescent probes

KW - Molecular devices

KW - Proteins

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A protein-driven DNA device that measures the excess binding energy of proteins that distort DNA

Abstract

Keywords

ASJC Scopus subject areas

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