A public genome-scale lentiviral expression library of human ORFs

Xiaoping Yang; Jesse S. Boehm; Xinping Yang; Kourosh Salehi-Ashtiani; Tong Hao; Yun Shen; Rakela Lubonja; Sapana R. Thomas; Ozan Alkan; Tashfeen Bhimdi; Thomas M. Green; Cory M. Johannessen; Serena J. Silver; Cindy Nguyen; Ryan R. Murray; Haley Hieronymus; Dawit Balcha; Changyu Fan; Chenwei Lin; Lila Ghamsari; Marc Vidal; William C. Hahn; David E. Hill; David E. Root

doi:10.1038/nmeth.1638

A public genome-scale lentiviral expression library of human ORFs

Xiaoping Yang, Jesse S. Boehm, Xinping Yang, Kourosh Salehi-Ashtiani, Tong Hao, Yun Shen, Rakela Lubonja, Sapana R. Thomas, Ozan Alkan, Tashfeen Bhimdi, Thomas M. Green, Cory M. Johannessen, Serena J. Silver, Cindy Nguyen, Ryan R. Murray, Haley Hieronymus, Dawit Balcha, Changyu Fan, Chenwei Lin, Lila GhamsariMarc Vidal, William C. Hahn, David E. Hill, David E. Root

Research output: Contribution to journal › Article › peer-review

Abstract

Functional characterization of the human genome requires tools for systematically modulating gene expression in both loss-of-function and gain-of-function experiments. We describe the production of a sequence-confirmed, clonal collection of over 16,100 human open-reading frames (ORFs) encoded in a versatile Gateway vector system. Using this ORFeome resource, we created a genome-scale expression collection in a lentiviral vector, thereby enabling both targeted experiments and high-throughput screens in diverse cell types.

Original language	English (US)
Pages (from-to)	659-661
Number of pages	3
Journal	Nature methods
Volume	8
Issue number	8
DOIs	https://doi.org/10.1038/nmeth.1638
State	Published - Aug 2011

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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Yang, X., Boehm, J. S., Yang, X., Salehi-Ashtiani, K., Hao, T., Shen, Y., Lubonja, R., Thomas, S. R., Alkan, O., Bhimdi, T., Green, T. M., Johannessen, C. M., Silver, S. J., Nguyen, C., Murray, R. R., Hieronymus, H., Balcha, D., Fan, C., Lin, C., ... Root, D. E. (2011). A public genome-scale lentiviral expression library of human ORFs. Nature methods, 8(8), 659-661. https://doi.org/10.1038/nmeth.1638

Yang, X, Boehm, JS, Yang, X, Salehi-Ashtiani, K, Hao, T, Shen, Y, Lubonja, R, Thomas, SR, Alkan, O, Bhimdi, T, Green, TM, Johannessen, CM, Silver, SJ, Nguyen, C, Murray, RR, Hieronymus, H, Balcha, D, Fan, C, Lin, C, Ghamsari, L, Vidal, M, Hahn, WC, Hill, DE & Root, DE 2011, 'A public genome-scale lentiviral expression library of human ORFs', Nature methods, vol. 8, no. 8, pp. 659-661. https://doi.org/10.1038/nmeth.1638

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title = "A public genome-scale lentiviral expression library of human ORFs",

abstract = "Functional characterization of the human genome requires tools for systematically modulating gene expression in both loss-of-function and gain-of-function experiments. We describe the production of a sequence-confirmed, clonal collection of over 16,100 human open-reading frames (ORFs) encoded in a versatile Gateway vector system. Using this ORFeome resource, we created a genome-scale expression collection in a lentiviral vector, thereby enabling both targeted experiments and high-throughput screens in diverse cell types.",

author = "Xiaoping Yang and Boehm, {Jesse S.} and Xinping Yang and Kourosh Salehi-Ashtiani and Tong Hao and Yun Shen and Rakela Lubonja and Thomas, {Sapana R.} and Ozan Alkan and Tashfeen Bhimdi and Green, {Thomas M.} and Johannessen, {Cory M.} and Silver, {Serena J.} and Cindy Nguyen and Murray, {Ryan R.} and Haley Hieronymus and Dawit Balcha and Changyu Fan and Chenwei Lin and Lila Ghamsari and Marc Vidal and Hahn, {William C.} and Hill, {David E.} and Root, {David E.}",

note = "Funding Information: We thank B. Piqani, I. Budianto, D. Szeto, T. Hirozane-Kishikawa, V. Swearingen, A. MacWilliams, T. Nieland, S. Hoang, J. Bochicchio, S. Young, A. Berlin, C. Russ, M. Garber and members of the Broad Institute Genetic Sequencing Platform who provided technical assistance or advice throughout this project, and J. Zhao, T. Roberts and T. Golub for participation in kinase ORFs subcollection generation. This work was supported by Broad Institute Scientific Planning and Allocation of Resources Committee funding, The Ellison Foundation (D.E.H. and M.V.), Dana-Farber Cancer Institute sponsored research funds to CCSB and Center for Cancer Genome Discovery and US National Institutes of Health R33 CA128625 (W.C.H., D.E.R. and D.E.H.).",

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month = aug,

doi = "10.1038/nmeth.1638",

language = "English (US)",

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AU - Boehm, Jesse S.

AU - Yang, Xinping

AU - Salehi-Ashtiani, Kourosh

AU - Hao, Tong

AU - Shen, Yun

AU - Lubonja, Rakela

AU - Thomas, Sapana R.

AU - Alkan, Ozan

AU - Bhimdi, Tashfeen

AU - Green, Thomas M.

AU - Johannessen, Cory M.

AU - Silver, Serena J.

AU - Nguyen, Cindy

AU - Murray, Ryan R.

AU - Hieronymus, Haley

AU - Balcha, Dawit

AU - Fan, Changyu

AU - Lin, Chenwei

AU - Ghamsari, Lila

AU - Vidal, Marc

AU - Hahn, William C.

AU - Hill, David E.

AU - Root, David E.

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A public genome-scale lentiviral expression library of human ORFs

Abstract

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