A public genome-scale lentiviral expression library of human ORFs

Xiaoping Yang, Jesse S. Boehm, Xinping Yang, Kourosh Salehi-Ashtiani, Tong Hao, Yun Shen, Rakela Lubonja, Sapana R. Thomas, Ozan Alkan, Tashfeen Bhimdi, Thomas M. Green, Cory M. Johannessen, Serena J. Silver, Cindy Nguyen, Ryan R. Murray, Haley Hieronymus, Dawit Balcha, Changyu Fan, Chenwei Lin, Lila GhamsariMarc Vidal, William C. Hahn, David E. Hill, David E. Root

Research output: Contribution to journalArticle

Abstract

Functional characterization of the human genome requires tools for systematically modulating gene expression in both loss-of-function and gain-of-function experiments. We describe the production of a sequence-confirmed, clonal collection of over 16,100 human open-reading frames (ORFs) encoded in a versatile Gateway vector system. Using this ORFeome resource, we created a genome-scale expression collection in a lentiviral vector, thereby enabling both targeted experiments and high-throughput screens in diverse cell types.

Original languageEnglish (US)
Pages (from-to)659-661
Number of pages3
JournalNature methods
Volume8
Issue number8
DOIs
StatePublished - Aug 2011

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Yang, X., Boehm, J. S., Yang, X., Salehi-Ashtiani, K., Hao, T., Shen, Y., Lubonja, R., Thomas, S. R., Alkan, O., Bhimdi, T., Green, T. M., Johannessen, C. M., Silver, S. J., Nguyen, C., Murray, R. R., Hieronymus, H., Balcha, D., Fan, C., Lin, C., ... Root, D. E. (2011). A public genome-scale lentiviral expression library of human ORFs. Nature methods, 8(8), 659-661. https://doi.org/10.1038/nmeth.1638