A ratiometric lectin microarray approach to analysis of the dynamic mammalian glycome

Kanoelani T. Pilobello; Deepika E. Slawek; Lara K. Mahal

doi:10.1073/pnas.0704954104

A ratiometric lectin microarray approach to analysis of the dynamic mammalian glycome

Kanoelani T. Pilobello, Deepika E. Slawek, Lara K. Mahal

Chemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Glycosylation creates an intricate and complex code for biological information that plays a role in cell-cell communication, infection, and immunity among many biological events. Dynamic changes in the glycosylation status of cells have been observed in tumor cell metastasis and cell differentiation but have been difficult to analyze because of a lack of high-throughput and facile technologies. Here, we present a method for the rapid evaluation of differences in the glycosylation of heterogeneous mammalian samples using a ratiometric two-color lectin microarray approach. This work represents a significant improvement in glycomics technology and sets the stage for the systematic evaluation of how glycans encode biological information in complex systems.

Original language	English (US)
Pages (from-to)	11534-11539
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	104
Issue number	28
DOIs	https://doi.org/10.1073/pnas.0704954104
State	Published - Jul 10 2007

Keywords

Carbohydrate analysis
Glycomics

ASJC Scopus subject areas

General

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abstract = "Glycosylation creates an intricate and complex code for biological information that plays a role in cell-cell communication, infection, and immunity among many biological events. Dynamic changes in the glycosylation status of cells have been observed in tumor cell metastasis and cell differentiation but have been difficult to analyze because of a lack of high-throughput and facile technologies. Here, we present a method for the rapid evaluation of differences in the glycosylation of heterogeneous mammalian samples using a ratiometric two-color lectin microarray approach. This work represents a significant improvement in glycomics technology and sets the stage for the systematic evaluation of how glycans encode biological information in complex systems.",

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AU - Slawek, Deepika E.

AU - Mahal, Lara K.

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Y1 - 2007/7/10

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AB - Glycosylation creates an intricate and complex code for biological information that plays a role in cell-cell communication, infection, and immunity among many biological events. Dynamic changes in the glycosylation status of cells have been observed in tumor cell metastasis and cell differentiation but have been difficult to analyze because of a lack of high-throughput and facile technologies. Here, we present a method for the rapid evaluation of differences in the glycosylation of heterogeneous mammalian samples using a ratiometric two-color lectin microarray approach. This work represents a significant improvement in glycomics technology and sets the stage for the systematic evaluation of how glycans encode biological information in complex systems.

KW - Carbohydrate analysis

KW - Glycomics

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A ratiometric lectin microarray approach to analysis of the dynamic mammalian glycome

Abstract

Keywords

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