@article{017163c9046b4fffae8a44c14bd93c81,
title = "A robust toolkit for functional profiling of the yeast genome",
abstract = "Study of mutant phenotypes is a fundamental method for understanding gene function. The construction of a near-complete collection of yeast knockouts (YKO) and the unique molecular barcodes (or TAGs) that identify each strain has enabled quantitative functional profiling of Saccharomyces cerevisiae. By using these TAGs and the SGA reporter, MFA1pr-HIS3, which facilitates conversion of heterozygous diploid YKO strains into haploid mutants, we have developed a set of highly efficient microarray-based techniques, collectively referred as dSLAM (diploid-based synthetic lethality analysis on microarrays), to probe genome-wide gene-chemical and gene-gene interactions. Direct comparison revealed that these techniques are more robust than existing methods in functional profiling of the yeast genome. Widespread application of these tools will elucidate a comprehensive yeast genetic network.",
author = "Xuewen Pan and Yuan, {Daniel S.} and Dong Xiang and Xiaoling Wang and Sharon Sookhai-Mahadeo and Bader, {Joel S.} and Philip Hieter and Forrest Spencer and Boeke, {Jef D.}",
note = "Funding Information: We are grateful to Dr. Siew Loon Ooi for constructing the URA3 marker (pSO142-4) used in creating the query constructs for synthetic interaction screens and valuable discussions. We thank Charlie Boone for providing the SGA reporter before publication and for helpful discussions and advice. We thank Maria Longese for sharing pML260 (YCplac111-GAL1pr-LCD1). We also thank R. Irizarry and Boeke and Spencer lab members for extensive discussions. X.P. is supported in part by a Leukemia & Lymphoma Society Fellowship. D.S.Y. was supported in part by the Burroughs-Wellcome Program in Computational Biology at Johns Hopkins and by a Kirschstein fellowship from the NIH (1F33HG002643-01A1). J.S.B. is supported in part by the Whitaker Foundation. This work was supported in part by NIH grants HG2432 and CA16519. ",
year = "2004",
month = nov,
day = "5",
doi = "10.1016/j.molcel.2004.09.035",
language = "English (US)",
volume = "16",
pages = "487--496",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "3",
}