A set of BAC clones spanning the human genome

Martin Krzywinski; Ian Bosdet; Duane Smailus; Readman Chiu; Carrie Mathewson; Natasja Wye; Sarah Barber; Mabel Brown-John; Susanna Chan; Steve Chand; Alison Cloutier; Noreen Girn; Darlene Lee; Amara Masson; Michael Mayo; Teika Olson; Pawan Pandoh; Anna Liisa Prabhu; Eric Schoenmakers; Miranda Tsai; Donna Albertson; Wan Lam; Chik On Choy; Kazutoyo Osoegawa; Shaying Zhao; Pieter J. de Jong; Jacqueline Schein; Steven Jones; Marco A. Marra

doi:10.1093/nar/gkh700

A set of BAC clones spanning the human genome

Martin Krzywinski, Ian Bosdet, Duane Smailus, Readman Chiu, Carrie Mathewson, Natasja Wye, Sarah Barber, Mabel Brown-John, Susanna Chan, Steve Chand, Alison Cloutier, Noreen Girn, Darlene Lee, Amara Masson, Michael Mayo, Teika Olson, Pawan Pandoh, Anna Liisa Prabhu, Eric Schoenmakers, Miranda TsaiDonna Albertson, Wan Lam, Chik On Choy, Kazutoyo Osoegawa, Shaying Zhao, Pieter J. de Jong, Jacqueline Schein, Steven Jones, Marco A. Marra

Oral and Maxillofacial Surgery

Research output: Contribution to journal › Article › peer-review

Abstract

Using the human bacterial artificial chromosome (BAC) fingerprint-based physical map, genome sequence assembly and BAC end sequences, we have generated a fingerprint-validated set of 32 855 BAC clones spanning the human genome. The clone set provides coverage for at least 98% of the human fingerprint map, 99% of the current assembled sequence and has an effective resolving power of 79 kb. We have made the clone set publicly available, anticipating that it will generally facilitate FISH or array-CGH-based identification and characterization of chromosomal alterations relevant to disease.

Original language	English (US)
Pages (from-to)	3651-3660
Number of pages	10
Journal	Nucleic acids research
Volume	32
Issue number	12
DOIs	https://doi.org/10.1093/nar/gkh700
State	Published - 2004

ASJC Scopus subject areas

Genetics

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Krzywinski, M., Bosdet, I., Smailus, D., Chiu, R., Mathewson, C., Wye, N., Barber, S., Brown-John, M., Chan, S., Chand, S., Cloutier, A., Girn, N., Lee, D., Masson, A., Mayo, M., Olson, T., Pandoh, P., Prabhu, A. L., Schoenmakers, E., ... Marra, M. A. (2004). A set of BAC clones spanning the human genome. Nucleic acids research, 32(12), 3651-3660. https://doi.org/10.1093/nar/gkh700

Krzywinski, M, Bosdet, I, Smailus, D, Chiu, R, Mathewson, C, Wye, N, Barber, S, Brown-John, M, Chan, S, Chand, S, Cloutier, A, Girn, N, Lee, D, Masson, A, Mayo, M, Olson, T, Pandoh, P, Prabhu, AL, Schoenmakers, E, Tsai, M, Albertson, D, Lam, W, Choy, CO, Osoegawa, K, Zhao, S, de Jong, PJ, Schein, J, Jones, S & Marra, MA 2004, 'A set of BAC clones spanning the human genome', Nucleic acids research, vol. 32, no. 12, pp. 3651-3660. https://doi.org/10.1093/nar/gkh700

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abstract = "Using the human bacterial artificial chromosome (BAC) fingerprint-based physical map, genome sequence assembly and BAC end sequences, we have generated a fingerprint-validated set of 32 855 BAC clones spanning the human genome. The clone set provides coverage for at least 98% of the human fingerprint map, 99% of the current assembled sequence and has an effective resolving power of 79 kb. We have made the clone set publicly available, anticipating that it will generally facilitate FISH or array-CGH-based identification and characterization of chromosomal alterations relevant to disease.",

author = "Martin Krzywinski and Ian Bosdet and Duane Smailus and Readman Chiu and Carrie Mathewson and Natasja Wye and Sarah Barber and Mabel Brown-John and Susanna Chan and Steve Chand and Alison Cloutier and Noreen Girn and Darlene Lee and Amara Masson and Michael Mayo and Teika Olson and Pawan Pandoh and Prabhu, {Anna Liisa} and Eric Schoenmakers and Miranda Tsai and Donna Albertson and Wan Lam and Choy, {Chik On} and Kazutoyo Osoegawa and Shaying Zhao and {de Jong}, {Pieter J.} and Jacqueline Schein and Steven Jones and Marra, {Marco A.}",

note = "Funding Information: We acknowledge useful discussions involving Dr Calum MacAulay and Dr Dan Pinkel, who encouraged us to move forward with a clone set spanning the human genome. M.A.M. and S.J. are scholars of the Michael Smith Foundation for Health Research. The authors wish to acknowledge support from the BC Cancer Foundation, Genome British Columbia, Genome Canada and the National Human Genome Research Institute (USA). The work presented here was funded by a Genome British Columbia Grant (Cancer Genomics) to Victor Ling, Connie Eaves and M.A.M.",

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doi = "10.1093/nar/gkh700",

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pages = "3651--3660",

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AU - Krzywinski, Martin

AU - Bosdet, Ian

AU - Smailus, Duane

AU - Chiu, Readman

AU - Mathewson, Carrie

AU - Wye, Natasja

AU - Barber, Sarah

AU - Brown-John, Mabel

AU - Chan, Susanna

AU - Chand, Steve

AU - Cloutier, Alison

AU - Girn, Noreen

AU - Lee, Darlene

AU - Masson, Amara

AU - Mayo, Michael

AU - Olson, Teika

AU - Pandoh, Pawan

AU - Prabhu, Anna Liisa

AU - Schoenmakers, Eric

AU - Tsai, Miranda

AU - Albertson, Donna

AU - Lam, Wan

AU - Choy, Chik On

AU - Osoegawa, Kazutoyo

AU - Zhao, Shaying

AU - de Jong, Pieter J.

AU - Schein, Jacqueline

AU - Jones, Steven

AU - Marra, Marco A.

N1 - Funding Information: We acknowledge useful discussions involving Dr Calum MacAulay and Dr Dan Pinkel, who encouraged us to move forward with a clone set spanning the human genome. M.A.M. and S.J. are scholars of the Michael Smith Foundation for Health Research. The authors wish to acknowledge support from the BC Cancer Foundation, Genome British Columbia, Genome Canada and the National Human Genome Research Institute (USA). The work presented here was funded by a Genome British Columbia Grant (Cancer Genomics) to Victor Ling, Connie Eaves and M.A.M.

PY - 2004

Y1 - 2004

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A set of BAC clones spanning the human genome

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