A single early-in-life macrolide course has lasting effects on murine microbial network topology and immunity

Victoria E. Ruiz; Thomas Battaglia; Zachary D. Kurtz; Luc Bijnens; Amy Ou; Isak Engstrand; Xuhui Zheng; Tadasu Iizumi; Briana J. Mullins; Christian L. Müller; Ken Cadwell; Richard Bonneau; Guillermo I. Perez-Perez; Martin J. Blaser

doi:10.1038/s41467-017-00531-6

A single early-in-life macrolide course has lasting effects on murine microbial network topology and immunity

Victoria E. Ruiz, Thomas Battaglia, Zachary D. Kurtz, Luc Bijnens, Amy Ou, Isak Engstrand, Xuhui Zheng, Tadasu Iizumi, Briana J. Mullins, Christian L. Müller, Ken Cadwell, Richard Bonneau, Guillermo I. Perez-Perez, Martin J. Blaser

Research output: Contribution to journal › Article › peer-review

Abstract

Broad-spectrum antibiotics are frequently prescribed to children. Early childhood represents a dynamic period for the intestinal microbial ecosystem, which is readily shaped by environmental cues; antibiotic-induced disruption of this sensitive community may have long-lasting host consequences. Here we demonstrate that a single pulsed macrolide antibiotic treatment (PAT) course early in life is sufficient to lead to durable alterations to the murine intestinal microbiota, ileal gene expression, specific intestinal T-cell populations, and secretory IgA expression. A PAT-perturbed microbial community is necessary for host effects and sufficient to transfer delayed secretory IgA expression. Additionally, early-life antibiotic exposure has lasting and transferable effects on microbial community network topology. Our results indicate that a single early-life macrolide course can alter the microbiota and modulate host immune phenotypes that persist long after exposure has ceased.

Original language	English (US)
Article number	518
Journal	Nature communications
Volume	8
Issue number	1
DOIs	https://doi.org/10.1038/s41467-017-00531-6
State	Published - Dec 1 2017

ASJC Scopus subject areas

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy

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Ruiz, V. E., Battaglia, T., Kurtz, Z. D., Bijnens, L., Ou, A., Engstrand, I., Zheng, X., Iizumi, T., Mullins, B. J., Müller, C. L., Cadwell, K., Bonneau, R., Perez-Perez, G. I., & Blaser, M. J. (2017). A single early-in-life macrolide course has lasting effects on murine microbial network topology and immunity. Nature communications, 8(1), Article 518. https://doi.org/10.1038/s41467-017-00531-6

Ruiz, VE, Battaglia, T, Kurtz, ZD, Bijnens, L, Ou, A, Engstrand, I, Zheng, X, Iizumi, T, Mullins, BJ, Müller, CL, Cadwell, K, Bonneau, R, Perez-Perez, GI & Blaser, MJ 2017, 'A single early-in-life macrolide course has lasting effects on murine microbial network topology and immunity', Nature communications, vol. 8, no. 1, 518. https://doi.org/10.1038/s41467-017-00531-6

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abstract = "Broad-spectrum antibiotics are frequently prescribed to children. Early childhood represents a dynamic period for the intestinal microbial ecosystem, which is readily shaped by environmental cues; antibiotic-induced disruption of this sensitive community may have long-lasting host consequences. Here we demonstrate that a single pulsed macrolide antibiotic treatment (PAT) course early in life is sufficient to lead to durable alterations to the murine intestinal microbiota, ileal gene expression, specific intestinal T-cell populations, and secretory IgA expression. A PAT-perturbed microbial community is necessary for host effects and sufficient to transfer delayed secretory IgA expression. Additionally, early-life antibiotic exposure has lasting and transferable effects on microbial community network topology. Our results indicate that a single early-life macrolide course can alter the microbiota and modulate host immune phenotypes that persist long after exposure has ceased.",

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AU - Ruiz, Victoria E.

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AU - Bijnens, Luc

AU - Ou, Amy

AU - Engstrand, Isak

AU - Zheng, Xuhui

AU - Iizumi, Tadasu

AU - Mullins, Briana J.

AU - Müller, Christian L.

AU - Cadwell, Ken

AU - Bonneau, Richard

AU - Perez-Perez, Guillermo I.

AU - Blaser, Martin J.

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AB - Broad-spectrum antibiotics are frequently prescribed to children. Early childhood represents a dynamic period for the intestinal microbial ecosystem, which is readily shaped by environmental cues; antibiotic-induced disruption of this sensitive community may have long-lasting host consequences. Here we demonstrate that a single pulsed macrolide antibiotic treatment (PAT) course early in life is sufficient to lead to durable alterations to the murine intestinal microbiota, ileal gene expression, specific intestinal T-cell populations, and secretory IgA expression. A PAT-perturbed microbial community is necessary for host effects and sufficient to transfer delayed secretory IgA expression. Additionally, early-life antibiotic exposure has lasting and transferable effects on microbial community network topology. Our results indicate that a single early-life macrolide course can alter the microbiota and modulate host immune phenotypes that persist long after exposure has ceased.

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A single early-in-life macrolide course has lasting effects on murine microbial network topology and immunity

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