TY - GEN
T1 - A single pressure pulse-actuated 3D-printed microfluidic tip for high throughput dispensing of C. elegans worms
AU - Chaturvedi, Nandita
AU - Baban, Navajit S.
AU - Sofela, Samuel O.
AU - Orozaliev, Ajymurat
AU - Giakoumidis, Nikolas
AU - Kim, Jongmin
AU - Gunsalus, Kristin C.
AU - Song, Yong Ak
N1 - Publisher Copyright:
Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - While C. elegans worms have been widely accepted as model organisms, isolating a controlled number of worms continues to be tedious. We have a devised a simple, easy-to-use mechanism for high-throughput dispensing of worms, by using a 3D-printed, microfluidic “dispensing” tip. Automating the fabrication and actuating the dispensing with single pressure pump pulses lend reliability to the setup, while integrating fully automated, 3-axis stages and an imaging platform enhance the rate of delivery. Furthermore, with simulational and experimental testing, we were able to identify the right geometry and pressure conditions for dispensing a single worm into a droplet as well.
AB - While C. elegans worms have been widely accepted as model organisms, isolating a controlled number of worms continues to be tedious. We have a devised a simple, easy-to-use mechanism for high-throughput dispensing of worms, by using a 3D-printed, microfluidic “dispensing” tip. Automating the fabrication and actuating the dispensing with single pressure pump pulses lend reliability to the setup, while integrating fully automated, 3-axis stages and an imaging platform enhance the rate of delivery. Furthermore, with simulational and experimental testing, we were able to identify the right geometry and pressure conditions for dispensing a single worm into a droplet as well.
KW - 3D-printed
KW - C. elegans
KW - Dispensing
KW - High-throughput
KW - Microfluidic
UR - http://www.scopus.com/inward/record.url?scp=85079750498&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85079750498&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85079750498
T3 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
SP - 1557
EP - 1559
BT - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PB - Chemical and Biological Microsystems Society
T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Y2 - 11 November 2018 through 15 November 2018
ER -