A single pressure pulse-actuated 3D-printed microfluidic tip for high throughput dispensing of C. elegans worms

Nandita Chaturvedi, Navajit S. Baban, Samuel O. Sofela, Ajymurat Orozaliev, Nikolas Giakoumidis, Jongmin Kim, Kristin C. Gunsalus, Yong Ak Song

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

While C. elegans worms have been widely accepted as model organisms, isolating a controlled number of worms continues to be tedious. We have a devised a simple, easy-to-use mechanism for high-throughput dispensing of worms, by using a 3D-printed, microfluidic “dispensing” tip. Automating the fabrication and actuating the dispensing with single pressure pump pulses lend reliability to the setup, while integrating fully automated, 3-axis stages and an imaging platform enhance the rate of delivery. Furthermore, with simulational and experimental testing, we were able to identify the right geometry and pressure conditions for dispensing a single worm into a droplet as well.

Original languageEnglish (US)
Title of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PublisherChemical and Biological Microsystems Society
Pages1557-1559
Number of pages3
ISBN (Electronic)9781510897571
StatePublished - Jan 1 2018
Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan, Province of China
Duration: Nov 11 2018Nov 15 2018

Publication series

Name22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Volume3

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
CountryTaiwan, Province of China
CityKaohsiung
Period11/11/1811/15/18

Keywords

  • 3D-printed
  • C. elegans
  • Dispensing
  • High-throughput
  • Microfluidic

ASJC Scopus subject areas

  • Chemistry(all)
  • Bioengineering
  • Chemical Engineering (miscellaneous)
  • Control and Systems Engineering

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