A small-molecule modulator of poly-α2,8-sialic acid expression on cultured neurons and tumor cells

L. K. Mahal, N. W. Charter, K. Angata, M. Fukuda, Jr Koshland, C. R. Bertozzi

Research output: Contribution to journalArticlepeer-review

Abstract

Poly-α2,8-sialic acid (PSA) has been implicated in numerous normal and pathological processes, including development, neuronal plasticity, and tumor metastasis. We report that cell surface PSA expression can be reversibly inhibited by a small molecule, N-butanoylmannosamine (ManBut). Inhibition occurs through a metabolic mechanism in which ManBut is converted to unnatural sialic acid derivatives that effectively act as chain terminators during cellular PSA biosynthesis. N-Propanoylmannosamine (ManProp), which differs from ManBut by a single methylene group, did not inhibit PSA biosynthesis. Modulation of PSA expression by chemical means has a role complementary to genetic and biochemical approaches in the study of complex PSA-mediated events.

Original languageEnglish (US)
Pages (from-to)380-382
Number of pages3
JournalScience
Volume294
Issue number5541
DOIs
StatePublished - Oct 12 2001

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'A small-molecule modulator of poly-α2,8-sialic acid expression on cultured neurons and tumor cells'. Together they form a unique fingerprint.

Cite this