TY - JOUR
T1 - A timer-actuated immunoassay cassette for detecting molecular markers in oral fluids
AU - Liu, Changchun
AU - Qiu, Xianbo
AU - Ongagna, Serge
AU - Chen, Dafeng
AU - Chen, Zongyuan
AU - Abrams, William R.
AU - Malamud, Daniel
AU - Corstjens, Paul L.A.M.
AU - Bau, Haim H.
PY - 2009
Y1 - 2009
N2 - An inexpensive, hand-held, point-of-care, disposable, self-contained immunoassay cassette comprised of air pouches for pumping, a metering chamber, reagents storage chambers, a mixer, and a lateral flow strip was designed, constructed, and tested. The assay was carried out in a consecutive flow format. The detection was facilitated with up-converting phosphor (UCP) reporter particles. The automated, timely pumping of the various reagents was driven by a spring-loaded timer. The utility of the cassette was demonstrated by detecting antibodies to HIV in saliva samples and further evaluated with a non-contagious, haptenized DNA assay. The cassette has several advantages over dip sticks such as sample preprocessing, integrated storage of reagents, and automated operation that reduces operator errors and training. The cassette and actuator described herein can readily be extended to detect biomarkers of other diseases in body fluids and other fluids at the point of care. The system is particularly suitable for resource-poor countries, where funds and trained personnel are in short supply.
AB - An inexpensive, hand-held, point-of-care, disposable, self-contained immunoassay cassette comprised of air pouches for pumping, a metering chamber, reagents storage chambers, a mixer, and a lateral flow strip was designed, constructed, and tested. The assay was carried out in a consecutive flow format. The detection was facilitated with up-converting phosphor (UCP) reporter particles. The automated, timely pumping of the various reagents was driven by a spring-loaded timer. The utility of the cassette was demonstrated by detecting antibodies to HIV in saliva samples and further evaluated with a non-contagious, haptenized DNA assay. The cassette has several advantages over dip sticks such as sample preprocessing, integrated storage of reagents, and automated operation that reduces operator errors and training. The cassette and actuator described herein can readily be extended to detect biomarkers of other diseases in body fluids and other fluids at the point of care. The system is particularly suitable for resource-poor countries, where funds and trained personnel are in short supply.
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U2 - 10.1039/b814322f
DO - 10.1039/b814322f
M3 - Article
C2 - 19255658
AN - SCOPUS:61849104523
SN - 1473-0197
VL - 9
SP - 768
EP - 776
JO - Lab on a Chip
JF - Lab on a Chip
IS - 6
ER -