A timer-actuated immunoassay cassette for detecting molecular markers in oral fluids

Changchun Liu, Xianbo Qiu, Serge Ongagna, Dafeng Chen, Zongyuan Chen, William R. Abrams, Daniel Malamud, Paul L.A.M. Corstjens, Haim H. Bau

Research output: Contribution to journalArticle

Abstract

An inexpensive, hand-held, point-of-care, disposable, self-contained immunoassay cassette comprised of air pouches for pumping, a metering chamber, reagents storage chambers, a mixer, and a lateral flow strip was designed, constructed, and tested. The assay was carried out in a consecutive flow format. The detection was facilitated with up-converting phosphor (UCP) reporter particles. The automated, timely pumping of the various reagents was driven by a spring-loaded timer. The utility of the cassette was demonstrated by detecting antibodies to HIV in saliva samples and further evaluated with a non-contagious, haptenized DNA assay. The cassette has several advantages over dip sticks such as sample preprocessing, integrated storage of reagents, and automated operation that reduces operator errors and training. The cassette and actuator described herein can readily be extended to detect biomarkers of other diseases in body fluids and other fluids at the point of care. The system is particularly suitable for resource-poor countries, where funds and trained personnel are in short supply.

Original languageEnglish (US)
Pages (from-to)768-776
Number of pages9
JournalLab on a Chip
Volume9
Issue number6
DOIs
StatePublished - 2009

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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