A transcription factor hierarchy defines an environmental stress response network

Liang Song; Shao Shan Carol Huang; Aaron Wise; Rosa Castanoz; Joseph R. Nery; Huaming Chen; Marina Watanabe; Jerushah Thomas; Ziv Bar-Joseph; Joseph R. Ecker

doi:10.1126/science.aag1550

A transcription factor hierarchy defines an environmental stress response network

Liang Song, Shao Shan Carol Huang, Aaron Wise, Rosa Castanoz, Joseph R. Nery, Huaming Chen, Marina Watanabe, Jerushah Thomas, Ziv Bar-Joseph, Joseph R. Ecker

Biology and Genomics

Research output: Contribution to journal › Article › peer-review

Abstract

Environmental stresses are universally encountered by microbes, plants, and animals.Yet systematic studies of stress-responsive transcription factor (TF) networks in multicellular organisms have been limited.The phytohormone abscisic acid (ABA) influences the expression of thousands of genes, allowing us to characterize complex stress-responsive regulatory networks. Using chromatin immunoprecipitation sequencing, we identified genome-wide targets of 21 ABA-related TFs to construct a comprehensive regulatory network in Arabidopsis thaliana. Determinants of dynamic TF binding and a hierarchy among TFs were defined, illuminating the relationship between differential gene expression patterns and ABA pathway feedback regulation. By extrapolating regulatory characteristics of observed canonical ABA pathway components, we identified a new family of transcriptional regulators modulating ABA and salt responsiveness and demonstrated their utility to modulate plant resilience to osmotic stress.

Original language	English (US)
Article number	aag1550
Journal	Science
Volume	354
Issue number	6312
DOIs	https://doi.org/10.1126/science.aag1550
State	Published - Nov 4 2016

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abstract = "Environmental stresses are universally encountered by microbes, plants, and animals.Yet systematic studies of stress-responsive transcription factor (TF) networks in multicellular organisms have been limited.The phytohormone abscisic acid (ABA) influences the expression of thousands of genes, allowing us to characterize complex stress-responsive regulatory networks. Using chromatin immunoprecipitation sequencing, we identified genome-wide targets of 21 ABA-related TFs to construct a comprehensive regulatory network in Arabidopsis thaliana. Determinants of dynamic TF binding and a hierarchy among TFs were defined, illuminating the relationship between differential gene expression patterns and ABA pathway feedback regulation. By extrapolating regulatory characteristics of observed canonical ABA pathway components, we identified a new family of transcriptional regulators modulating ABA and salt responsiveness and demonstrated their utility to modulate plant resilience to osmotic stress.",

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AU - Song, Liang

AU - Huang, Shao Shan Carol

AU - Wise, Aaron

AU - Castanoz, Rosa

AU - Nery, Joseph R.

AU - Chen, Huaming

AU - Watanabe, Marina

AU - Thomas, Jerushah

AU - Bar-Joseph, Ziv

AU - Ecker, Joseph R.

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A transcription factor hierarchy defines an environmental stress response network

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