A viral discovery methodology for clinical biopsy samples utilising massively parallel next generation sequencing

Gordon M. Daly, Nick Bexfield, Judith Heaney, Sam Stubbs, Antonia P. Mayer, Anne Palser, Paul Kellam, Nizar Drou, Mario Caccamo, Laurence Tiley, Graeme J.M. Alexander, William Bernal, Jonathan L. Heeney

Research output: Contribution to journalArticlepeer-review

Abstract

Here we describe a virus discovery protocol for a range of different virus genera, that can be applied to biopsy-sized tissue samples. Our viral enrichment procedure, validated using canine and human liver samples, significantly improves viral read copy number and increases the length of viral contigs that can be generated by de novo assembly. This in turn enables the Illumina next generation sequencing (NGS) platform to be used as an effective tool for viral discovery from tissue samples.

Original languageEnglish (US)
Article numbere28879
JournalPloS one
Volume6
Issue number12
DOIs
StatePublished - Dec 21 2011

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Fingerprint Dive into the research topics of 'A viral discovery methodology for clinical biopsy samples utilising massively parallel next generation sequencing'. Together they form a unique fingerprint.

Cite this