A viral strategy for targeting and manipulating interneurons across vertebrate species

Jordane Dimidschstein, Qian Chen, Robin Tremblay, Stephanie L. Rogers, Giuseppe Antonio Saldi, Lihua Guo, Qing Xu, Runpeng Liu, Congyi Lu, Jianhua Chu, Joshua S. Grimley, Anne Rachel Krostag, Ajamete Kaykas, Michael C. Avery, Mohammad S. Rashid, Myungin Baek, Amanda L. Jacob, Gordon B. Smith, Daniel E. Wilson, Georg KoscheIllya Kruglikov, Tomasz Rusielewicz, Vibhakar C. Kotak, Todd M. Mowery, Stewart A. Anderson, Edward M. Callaway, Jeremy S. Dasen, David Fitzpatrick, Valentina Fossati, Michael A. Long, Scott Noggle, John H. Reynolds, Dan H. Sanes, Bernardo Rudy, Guoping Feng, Gord Fishell

Research output: Contribution to journalArticlepeer-review


A fundamental impediment to understanding the brain is the availability of inexpensive and robust methods for targeting and manipulating specific neuronal populations. The need to overcome this barrier is pressing because there are considerable anatomical, physiological, cognitive and behavioral differences between mice and higher mammalian species in which it is difficult to specifically target and manipulate genetically defined functional cell types. In particular, it is unclear the degree to which insights from mouse models can shed light on the neural mechanisms that mediate cognitive functions in higher species, including humans. Here we describe a novel recombinant adeno-associated virus that restricts gene expression to GABAergic interneurons within the telencephalon. We demonstrate that the viral expression is specific and robust, allowing for morphological visualization, activity monitoring and functional manipulation of interneurons in both mice and non-genetically tractable species, thus opening the possibility to study GABAergic function in virtually any vertebrate species.

Original languageEnglish (US)
Pages (from-to)1743-1749
Number of pages7
JournalNature Neuroscience
Issue number12
StatePublished - Dec 1 2016

ASJC Scopus subject areas

  • General Neuroscience


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