A viral strategy for targeting and manipulating interneurons across vertebrate species

Jordane Dimidschstein; Qian Chen; Robin Tremblay; Stephanie L. Rogers; Giuseppe Antonio Saldi; Lihua Guo; Qing Xu; Runpeng Liu; Congyi Lu; Jianhua Chu; Joshua S. Grimley; Anne Rachel Krostag; Ajamete Kaykas; Michael C. Avery; Mohammad S. Rashid; Myungin Baek; Amanda L. Jacob; Gordon B. Smith; Daniel E. Wilson; Georg Kosche; Illya Kruglikov; Tomasz Rusielewicz; Vibhakar C. Kotak; Todd M. Mowery; Stewart A. Anderson; Edward M. Callaway; Jeremy S. Dasen; David Fitzpatrick; Valentina Fossati; Michael A. Long; Scott Noggle; John H. Reynolds; Dan H. Sanes; Bernardo Rudy; Guoping Feng; Gord Fishell

doi:10.1038/nn.4430

A viral strategy for targeting and manipulating interneurons across vertebrate species

Jordane Dimidschstein, Qian Chen, Robin Tremblay, Stephanie L. Rogers, Giuseppe Antonio Saldi, Lihua Guo, Qing Xu, Runpeng Liu, Congyi Lu, Jianhua Chu, Joshua S. Grimley, Anne Rachel Krostag, Ajamete Kaykas, Michael C. Avery, Mohammad S. Rashid, Myungin Baek, Amanda L. Jacob, Gordon B. Smith, Daniel E. Wilson, Georg KoscheIllya Kruglikov, Tomasz Rusielewicz, Vibhakar C. Kotak, Todd M. Mowery, Stewart A. Anderson, Edward M. Callaway, Jeremy S. Dasen, David Fitzpatrick, Valentina Fossati, Michael A. Long, Scott Noggle, John H. Reynolds, Dan H. Sanes, Bernardo Rudy, Guoping Feng, Gord Fishell

Research output: Contribution to journal › Article › peer-review

Abstract

A fundamental impediment to understanding the brain is the availability of inexpensive and robust methods for targeting and manipulating specific neuronal populations. The need to overcome this barrier is pressing because there are considerable anatomical, physiological, cognitive and behavioral differences between mice and higher mammalian species in which it is difficult to specifically target and manipulate genetically defined functional cell types. In particular, it is unclear the degree to which insights from mouse models can shed light on the neural mechanisms that mediate cognitive functions in higher species, including humans. Here we describe a novel recombinant adeno-associated virus that restricts gene expression to GABAergic interneurons within the telencephalon. We demonstrate that the viral expression is specific and robust, allowing for morphological visualization, activity monitoring and functional manipulation of interneurons in both mice and non-genetically tractable species, thus opening the possibility to study GABAergic function in virtually any vertebrate species.

Original language	English (US)
Pages (from-to)	1743-1749
Number of pages	7
Journal	Nature Neuroscience
Volume	19
Issue number	12
DOIs	https://doi.org/10.1038/nn.4430
State	Published - Dec 1 2016

ASJC Scopus subject areas

General Neuroscience

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Dimidschstein, J., Chen, Q., Tremblay, R., Rogers, S. L., Saldi, G. A., Guo, L., Xu, Q., Liu, R., Lu, C., Chu, J., Grimley, J. S., Krostag, A. R., Kaykas, A., Avery, M. C., Rashid, M. S., Baek, M., Jacob, A. L., Smith, G. B., Wilson, D. E., ... Fishell, G. (2016). A viral strategy for targeting and manipulating interneurons across vertebrate species. Nature Neuroscience, 19(12), 1743-1749. https://doi.org/10.1038/nn.4430

Dimidschstein, J, Chen, Q, Tremblay, R, Rogers, SL, Saldi, GA, Guo, L, Xu, Q, Liu, R, Lu, C, Chu, J, Grimley, JS, Krostag, AR, Kaykas, A, Avery, MC, Rashid, MS, Baek, M, Jacob, AL, Smith, GB, Wilson, DE, Kosche, G, Kruglikov, I, Rusielewicz, T, Kotak, VC, Mowery, TM, Anderson, SA, Callaway, EM, Dasen, JS, Fitzpatrick, D, Fossati, V, Long, MA, Noggle, S, Reynolds, JH, Sanes, DH, Rudy, B, Feng, G & Fishell, G 2016, 'A viral strategy for targeting and manipulating interneurons across vertebrate species', Nature Neuroscience, vol. 19, no. 12, pp. 1743-1749. https://doi.org/10.1038/nn.4430

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AU - Guo, Lihua

AU - Xu, Qing

AU - Liu, Runpeng

AU - Lu, Congyi

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AU - Krostag, Anne Rachel

AU - Kaykas, Ajamete

AU - Avery, Michael C.

AU - Rashid, Mohammad S.

AU - Baek, Myungin

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AU - Smith, Gordon B.

AU - Wilson, Daniel E.

AU - Kosche, Georg

AU - Kruglikov, Illya

AU - Rusielewicz, Tomasz

AU - Kotak, Vibhakar C.

AU - Mowery, Todd M.

AU - Anderson, Stewart A.

AU - Callaway, Edward M.

AU - Dasen, Jeremy S.

AU - Fitzpatrick, David

AU - Fossati, Valentina

AU - Long, Michael A.

AU - Noggle, Scott

AU - Reynolds, John H.

AU - Sanes, Dan H.

AU - Rudy, Bernardo

AU - Feng, Guoping

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A viral strategy for targeting and manipulating interneurons across vertebrate species

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