Abstract
Absorption difference spectra between highly concentrated and dilute solutions of chlorophyll a have been measured in ethanol, maintaining the product of concentration and path length constant. A log-log plot of dimer versus monomer concentration, derived from the data, was linear with a slope of 2.0, which is consistent with the interpretation that dimers are present in this solvent. The absorption spectrum of the dimer in ethanol was calculated, and a value of 4.5 ± 0.8 M-1 was obtained for the equilibrium constant for dimerization.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 183-187 |
| Number of pages | 5 |
| Journal | BBA - Bioenergetics |
| Volume | 153 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 15 1968 |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Cell Biology
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Cite this
Broyde, S. B., Brody, S. S., & Brody, M. (1968). Absorption difference spectroscopy of chlorophyll a in ethanol solution. BBA - Bioenergetics, 153(1), 183-187. https://doi.org/10.1016/0005-2728(68)90158-8