Absorption difference spectroscopy of chlorophyll a in ethanol solution

S. B. Broyde; S. S. Brody; M. Brody

doi:10.1016/0005-2728(68)90158-8

Absorption difference spectroscopy of chlorophyll a in ethanol solution

S. B. Broyde, S. S. Brody, M. Brody

Biology and Genomics

Research output: Contribution to journal › Article › peer-review

Abstract

Absorption difference spectra between highly concentrated and dilute solutions of chlorophyll a have been measured in ethanol, maintaining the product of concentration and path length constant. A log-log plot of dimer versus monomer concentration, derived from the data, was linear with a slope of 2.0, which is consistent with the interpretation that dimers are present in this solvent. The absorption spectrum of the dimer in ethanol was calculated, and a value of 4.5 ± 0.8 M^-1 was obtained for the equilibrium constant for dimerization.

Original language	English (US)
Pages (from-to)	183-187
Number of pages	5
Journal	BBA - Bioenergetics
Volume	153
Issue number	1
DOIs	https://doi.org/10.1016/0005-2728(68)90158-8
State	Published - Jan 15 1968

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

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10.1016/0005-2728(68)90158-8

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title = "Absorption difference spectroscopy of chlorophyll a in ethanol solution",

abstract = "Absorption difference spectra between highly concentrated and dilute solutions of chlorophyll a have been measured in ethanol, maintaining the product of concentration and path length constant. A log-log plot of dimer versus monomer concentration, derived from the data, was linear with a slope of 2.0, which is consistent with the interpretation that dimers are present in this solvent. The absorption spectrum of the dimer in ethanol was calculated, and a value of 4.5 ± 0.8 M-1 was obtained for the equilibrium constant for dimerization.",

author = "Broyde, {S. B.} and Brody, {S. S.} and M. Brody",

note = "Funding Information: This research was carried out with the support of the National Science Foundation under Grant GB-4o78. S.S.B. was supported (in part) by a Public Health Service Research Career Program Award, GM 14747. We also wish to thank the University Computing Center for grants of time on the CDC 6600 computer at the Courant Institute of Mathematical Science at New York University.",

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doi = "10.1016/0005-2728(68)90158-8",

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AU - Brody, S. S.

AU - Brody, M.

N1 - Funding Information: This research was carried out with the support of the National Science Foundation under Grant GB-4o78. S.S.B. was supported (in part) by a Public Health Service Research Career Program Award, GM 14747. We also wish to thank the University Computing Center for grants of time on the CDC 6600 computer at the Courant Institute of Mathematical Science at New York University.

PY - 1968/1/15

Y1 - 1968/1/15

N2 - Absorption difference spectra between highly concentrated and dilute solutions of chlorophyll a have been measured in ethanol, maintaining the product of concentration and path length constant. A log-log plot of dimer versus monomer concentration, derived from the data, was linear with a slope of 2.0, which is consistent with the interpretation that dimers are present in this solvent. The absorption spectrum of the dimer in ethanol was calculated, and a value of 4.5 ± 0.8 M-1 was obtained for the equilibrium constant for dimerization.

AB - Absorption difference spectra between highly concentrated and dilute solutions of chlorophyll a have been measured in ethanol, maintaining the product of concentration and path length constant. A log-log plot of dimer versus monomer concentration, derived from the data, was linear with a slope of 2.0, which is consistent with the interpretation that dimers are present in this solvent. The absorption spectrum of the dimer in ethanol was calculated, and a value of 4.5 ± 0.8 M-1 was obtained for the equilibrium constant for dimerization.

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Absorption difference spectroscopy of chlorophyll a in ethanol solution

Abstract

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