Absorption difference spectroscopy of chlorophyll a in ethanol solution

S. B. Broyde, S. S. Brody, M. Brody

Research output: Contribution to journalArticlepeer-review

Abstract

Absorption difference spectra between highly concentrated and dilute solutions of chlorophyll a have been measured in ethanol, maintaining the product of concentration and path length constant. A log-log plot of dimer versus monomer concentration, derived from the data, was linear with a slope of 2.0, which is consistent with the interpretation that dimers are present in this solvent. The absorption spectrum of the dimer in ethanol was calculated, and a value of 4.5 ± 0.8 M-1 was obtained for the equilibrium constant for dimerization.

Original languageEnglish (US)
Pages (from-to)183-187
Number of pages5
JournalBBA - Bioenergetics
Volume153
Issue number1
DOIs
StatePublished - Jan 15 1968

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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