TY - GEN
T1 - Accelerating the mass transport of DNA biomolecules onto DNA microarray for enhanced detection by electrokinetic concentration in a microfluidic chip
AU - Martins, Diogo
AU - Wei, Xi
AU - Levicky, Rastislav
AU - Song, Yong Ak
N1 - Publisher Copyright:
© 2016 by ASME.
PY - 2016
Y1 - 2016
N2 - Morpholinos (MOs) are synthetic nucleic acids analogues with a non-charged backbone of morpholine rings. To enhance the MO-DNA hybridization assay speed, we propose the integration of a MO microarray with an ion concentration polarization (ICP) based microfluidic concentrator. The ICP concentrator collects target biomolecules from a ∼μL fluidic DNA sample and concentrates them electrokinetically into a ∼nL plug located in the vicinity of the MO probes. ICP preconcentration not only reduces the analyte diffusion length but also increases the binding reaction rate, and as a result, ICPenhanced MO microarrays allow much faster hybridization than standard diffusion-limited MO microarrays.
AB - Morpholinos (MOs) are synthetic nucleic acids analogues with a non-charged backbone of morpholine rings. To enhance the MO-DNA hybridization assay speed, we propose the integration of a MO microarray with an ion concentration polarization (ICP) based microfluidic concentrator. The ICP concentrator collects target biomolecules from a ∼μL fluidic DNA sample and concentrates them electrokinetically into a ∼nL plug located in the vicinity of the MO probes. ICP preconcentration not only reduces the analyte diffusion length but also increases the binding reaction rate, and as a result, ICPenhanced MO microarrays allow much faster hybridization than standard diffusion-limited MO microarrays.
UR - http://www.scopus.com/inward/record.url?scp=84968754982&partnerID=8YFLogxK
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U2 - 10.1115/MNHMT2016-6562
DO - 10.1115/MNHMT2016-6562
M3 - Conference contribution
AN - SCOPUS:84968754982
T3 - ASME 2016 5th International Conference on Micro/Nanoscale Heat and Mass Transfer, MNHMT 2016
BT - Micro/Nanofluidics and Lab-on-a-Chip; Nanofluids; Micro/Nanoscale Interfacial Transport Phenomena; Micro/Nanoscale Boiling and Condensation Heat Transfer; Micro/Nanoscale Thermal Radiation; Micro/Nanoscale Energy Devices and Systems
PB - American Society of Mechanical Engineers
T2 - ASME 2016 5th International Conference on Micro/Nanoscale Heat and Mass Transfer, MNHMT 2016
Y2 - 4 January 2016 through 6 January 2016
ER -