Accumulation of NO2-cobalamin in nutrient-stressed ammonia-oxidizing archaea and in the oxygen deficient zone of the eastern tropical North Pacific

Katherine R. Heal, Wei Qin, Shady A. Amin, Allan H. Devol, James W. Moffett, E. Virginia Armbrust, David A. Stahl, Anitra E. Ingalls

    Research output: Contribution to journalArticle

    Abstract

    Cobalamin (vitamin B12) is a precious resource in natural systems that is produced by select prokaryotes and required by a broad range of organisms. In this way, the production of cobalamin reinforces numerous microbial interdependencies. Here we report the accumulation of an unusual form of cobalamin, nitrocobalamin (NO2-cobalamin), in a marine oxygen deficient zone (ODZ), isolates of ammonia-oxidizing archaea (AOA), and an anaerobic ammonium-oxidizing (anammox) bacteria enriched bioreactor. Low oxygen waters were enriched in NO2-cobalamin, and AOA isolates experiencing ammonia or copper stress produced more NO2-cobalamin, though there is wide strain-to-strain and batch-to-batch variability. NO2-cobalamin has no known biochemical role. We hypothesize that AOA and anammox bacteria are a source of marine NO2-cobalamin in the environment via a reactive nitrogen intermediate. These findings suggest connections between cobalamin forms and nitrogen transformations, physiological stress and ocean deoxygenation.

    LanguageEnglish (US)
    Pages453-457
    Number of pages5
    JournalEnvironmental Microbiology Reports
    Volume10
    Issue number4
    DOIs
    StatePublished - Aug 1 2018

    Fingerprint

    Archaea
    vitamin B12
    Vitamin B 12
    Ammonia
    ammonia
    Oxygen
    oxygen
    Food
    nutrient
    nutrients
    ammonium
    bacterium
    nitrogen
    prokaryote
    microbiology
    vitamin
    bioreactor
    Ammonium Compounds
    copper
    Nitrogen

    ASJC Scopus subject areas

    • Ecology, Evolution, Behavior and Systematics
    • Agricultural and Biological Sciences (miscellaneous)

    Cite this

    Accumulation of NO2-cobalamin in nutrient-stressed ammonia-oxidizing archaea and in the oxygen deficient zone of the eastern tropical North Pacific. / Heal, Katherine R.; Qin, Wei; Amin, Shady A.; Devol, Allan H.; Moffett, James W.; Armbrust, E. Virginia; Stahl, David A.; Ingalls, Anitra E.

    In: Environmental Microbiology Reports, Vol. 10, No. 4, 01.08.2018, p. 453-457.

    Research output: Contribution to journalArticle

    Heal, Katherine R. ; Qin, Wei ; Amin, Shady A. ; Devol, Allan H. ; Moffett, James W. ; Armbrust, E. Virginia ; Stahl, David A. ; Ingalls, Anitra E./ Accumulation of NO2-cobalamin in nutrient-stressed ammonia-oxidizing archaea and in the oxygen deficient zone of the eastern tropical North Pacific. In: Environmental Microbiology Reports. 2018 ; Vol. 10, No. 4. pp. 453-457
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